School of Chemical Engineering, Ocean and Life Sciences, Dalian University of Technology, NO.2 Dagong Road, Panjin City, Liaoning Province, 124221, China.
School of Chemical Engineering, Ocean and Life Sciences, Dalian University of Technology, NO.2 Dagong Road, Panjin City, Liaoning Province, 124221, China.
Environ Pollut. 2024 Nov 15;361:124872. doi: 10.1016/j.envpol.2024.124872. Epub 2024 Sep 3.
Heterocapsa bohaiensis is a newly identified dinoflagellate species that causes harmful blooms in coastal areas in China, Malaysian, and New Caledonian. These blooms have led to substantial economic losses for local aquaculture. Previous studies have mainly focused on understanding the toxicity of H. bohaiensis. However, the causes of H. bohaiensis blooms remain unknown. In this study, we aimed to ascertain nitrogen (N) and phosphorus (P) requirements for the growth and reproduction of H. bohaiensis. Additionally, we sought to understand the functional mechanisms by comparing the transcriptomes of H. bohaiensis under nutrient-limited conditions and control conditions. The results revealed a wide range of acceptable N:P ratios for H. bohainensis, attributed to a mechanism involving nutrient storage, which allowed H. bohainensis to sustain its growth even when either nitrate or phosphate was depleted. Higher N:P ratios (>27.5) were more conducive to the growth of H. bohainensis than f/2 medium or low ratios, which is related to the N:P ratios absorbed by H. bohainensis. The toxicity of H. bohainensis was significantly enhanced in N-limited or P-limited states. These findings underscore the significance of the physiological metabolism of H. bohainensis in adapting to environmental stresses induced by human activities and establishing the dominance of blooms.
海洋卡盾藻是一种新鉴定的甲藻，在中国、马来西亚和新喀里多尼亚沿海地区引发有害水华。这些水华给当地水产养殖业造成了巨大的经济损失。先前的研究主要集中在了解海洋卡盾藻的毒性。然而，海洋卡盾藻水华的成因仍不清楚。在这项研究中，我们旨在确定海洋卡盾藻生长和繁殖所需的氮（N）和磷（P）。此外，我们通过比较营养限制条件和对照条件下海洋卡盾藻的转录组，试图了解其功能机制。结果表明，海洋卡盾藻对 N:P 比的适应范围很广，这归因于一种营养储存机制，即使硝酸盐或磷酸盐耗尽，海洋卡盾藻也能维持其生长。与 f/2 培养基或低 N:P 比相比，较高的 N:P 比（>27.5）更有利于海洋卡盾藻的生长，这与海洋卡盾藻吸收的 N:P 比有关。在氮限制或磷限制的状态下，海洋卡盾藻的毒性显著增强。这些发现强调了海洋卡盾藻适应人类活动引起的环境胁迫和建立水华优势的生理代谢的重要性。
