Department of Human Nutrition and Dietetics, Faculty of Food Science and Nutrition, Poznan University of Life Sciences, 60-624 Poznan, Poland.
Department of Biotechnology and Food Microbiology, Faculty of Food Science and Nutrition, Poznan University of Life Sciences, 60-637 Poznan, Poland.
Nutrients. 2024 Aug 2;16(15):2524. doi: 10.3390/nu16152524.
Osteoporosis in menopausal women requires alternatives to current medications, considering their adverse effects. In this context, probiotics and isoflavone products are promising dietary interventions. The objective of our study was to examine the impacts of and its combination with daidzein and tempeh on calcium status, calcium transporters, and bone metabolism biomarkers in a post-menopausal osteoporotic rat model. A total of 48 female Wistar rats were exposed to a two-stage experiment involving calcium deficit induction and subsequent dietary interventions across six groups. Calcium levels, the gene expression of TRPV5 and TRPV6 calcium transporters, bone histopathology, serum bone metabolism markers, and blood biochemistry were evaluated. The results revealed that, while decreasing serum calcium levels, the groups that received the probiotic and isoflavone combination exhibited increased bone metabolism biomarkers and decreased calcium transporter expressions, akin to the effects of bisphosphonate. Additionally, significant improvements in bone histopathology were observed in these groups. However, the group receiving probiotic alone did not exhibit significant changes in bone resorption biomarkers, calcium transporter expression, or various blood parameters. Meanwhile, the combination of probiotic with tempeh positively influenced hematological parameters and reduced cholesterol and triglyceride levels, but it led to elevated blood glucose levels. Correlation analyses highlighted associations between serum calcium levels, calcium transporter expression, and bone metabolism biomarkers. In conclusion, our findings suggest that the daily consumption of probiotic in combination with isoflavone products may improve bone health in ovariectomized rats, warranting further research to elucidate potential interactions with other nutrients.
绝经后妇女骨质疏松症需要替代当前药物，考虑到其不良反应。在这种情况下，益生菌和异黄酮产品是很有前途的饮食干预措施。我们的研究目的是研究 和其与大豆苷元和豆豉结合对绝经后骨质疏松症大鼠模型钙状态、钙转运蛋白和骨代谢生物标志物的影响。总共 48 只雌性 Wistar 大鼠经历了一个两阶段实验，涉及钙缺乏诱导和随后的六个组的饮食干预。评估了钙水平、TRPV5 和 TRPV6 钙转运体的基因表达、骨组织病理学、血清骨代谢标志物和血液生化。结果表明，虽然降低了血清钙水平，但接受益生菌 和异黄酮联合治疗的组表现出增加的骨代谢生物标志物和降低的钙转运体表达，类似于双膦酸盐的作用。此外，这些组的骨组织病理学得到了显著改善。然而，单独接受益生菌 的组在骨吸收生物标志物、钙转运体表达或各种血液参数方面没有显示出显著变化。同时，益生菌 与豆豉的联合使用对血液参数有积极影响，并降低了胆固醇和甘油三酯水平，但导致血糖水平升高。相关性分析强调了血清钙水平、钙转运体表达和骨代谢生物标志物之间的关联。总之，我们的研究结果表明，每天食用益生菌 和异黄酮产品可能会改善去卵巢大鼠的骨骼健康，需要进一步研究以阐明与其他营养素的潜在相互作用。
