Cheng Cong, Zhang Fengyuan, Li Li, Ni Zhiyong
Xinjiang Key Laboratory for Ecological Adaptation and Evolution of Extreme Environment Biology, College of Life Sciences, Xinjiang Agricultural University, Urumqi 830052, China.
College of Life Science, Xinjiang Agricultural University, Urumqi 830052, China.
Plants (Basel). 2024 Dec 16;13(24):3510. doi: 10.3390/plants13243510.
Plant plasma membrane (PM) H-ATPase functions as a proton-motive force by exporting cellular protons to establish a transmembrane chemical gradient of H ions and an accompanying electrical gradient. These gradients are crucial for plant growth and development and for plant responses to abiotic and biotic stresses. In this study, a comprehensive identification of the PM H-ATPase gene family was conducted across four cotton species. Specifically, 14 genes were identified in the diploid species and , whereas 39 and 43 genes were identified in the tetraploid species and , respectively. The characteristics of this gene family were subsequently compared and analyzed using bioinformatics. Chromosomal localization and collinearity analyses elucidated the distribution characteristics of this gene family within the cotton genomes. Gene structure and phylogenetic analyses demonstrated the conservation of this family across cotton species, whereas the examination of -acting elements in gene promoters highlighted their involvement in environmental stress and hormone response categories. An expression profile analysis revealed eight genes whose expression was upregulated under salt stress conditions, and quantitative real-time PCR results suggested that the cotton PM H-ATPase genes may play crucial roles in conferring resistance to salt stress. These findings establish a robust foundation for subsequent investigations into the functions of cotton PM H-ATPase genes and may offer valuable insights for selecting genes for resistance breeding programs.
植物质膜（PM）H⁺-ATP酶通过输出细胞质子以建立H⁺离子的跨膜化学梯度和伴随的电势梯度来发挥质子动力的作用。这些梯度对于植物的生长发育以及植物对非生物和生物胁迫的响应至关重要。在本研究中，对四个棉花物种的质膜H⁺-ATP酶基因家族进行了全面鉴定。具体而言，在二倍体物种和中分别鉴定出14个基因，而在四倍体物种和中分别鉴定出39个和43个基因。随后使用生物信息学对该基因家族的特征进行了比较和分析。染色体定位和共线性分析阐明了该基因家族在棉花基因组中的分布特征。基因结构和系统发育分析表明该家族在棉花物种间具有保守性，而对基因启动子中顺式作用元件的研究突出了它们参与环境胁迫和激素反应类别。表达谱分析揭示了八个在盐胁迫条件下表达上调的基因，定量实时PCR结果表明棉花质膜H⁺-ATP酶基因可能在赋予抗盐胁迫能力方面发挥关键作用。这些发现为后续对棉花质膜H⁺-ATP酶基因功能的研究奠定了坚实基础，并可能为抗性育种计划的基因选择提供有价值的见解。
