棉花FAH基因家族在盐胁迫下的全基因组分析与功能验证

Genome-wide analysis and functional validation of the cotton FAH gene family for salt stress.

作者信息

Gu Haijing, Feng Wenxiang, Mehari Teame Gereziher, Wang Yifan, Wang Ziyin, Xu Yifan, Zhao Yizhou, Tang Junfeng, Zhang Ke, Zhou Zitong, Wang Wei, Zhou Ruqin, Wu Jianyong, Wang Baohua

机构信息

School of Life Sciences, Nantong University, Nantong, Jiangsu, 226019, China.

State Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, 455000, China.

出版信息

BMC Genomics. 2025 Mar 18;26(1):271. doi: 10.1186/s12864-025-11450-y.

DOI:10.1186/s12864-025-11450-y

PMID:40102754

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11921677/

Abstract

BACKGROUND

Fatty acid hydroxylases (FAHs) are a family of enzymes that includes fatty acid hydroxylases, carotenoid hydroxylases, and sterol desaturases. Fatty acids are highly important for plants. They are the main source of energy storage and the main component of the cell membrane. Saturated fatty acids can be divided into two categories: saturated fatty acids and unsaturated fatty acids. FAHs play a pivotal role in enhancing plant salt tolerance by modulating fatty acid metabolic pathways, thereby improving cell membrane stability and antioxidant capacity.

RESULTS

In this study, we identified a total of 129 FAH gene family members in four cotton species, namely, Gossypium hirsutum, Gossypium darwinii, Gossypium arboreum, and Gossypium raimondii. The FAH genes were divided into five subgroups via evolutionary analysis. FAH genes located in the same subgroup presented similar gene structures and a consistent distribution of conserved motifs through the analysis of evolutionary trees, gene structures, and conserved motifs. Chromosomal localization analysis of the FAH gene family revealed that it has undergone chromosomal segment duplication events. Analysis of cis-acting elements suggested that the FAH gene may be involved in regulating biotic and abiotic stresses, plant growth and development, signaling pathways, and other physiological processes. The RT‒qPCR results revealed significant differences in the expression levels of FAH gene family members under salt stress conditions compared with those in the control group. Additionally, we successfully silenced Gohir.A03G045300 through VIGS experiments, and the results indicated that the silenced plants were more sensitive to salt stress than the control plants were. This suggests that Gohir.A03G045300 may be involved in the response of cotton to salt stress.

CONCLUSIONS

A total of 129 FAH genes were identified in four Gossypium species through bioinformatics analysis. Gene silencing of FAH members in G. hirsutum revealed that the FAH gene family plays a crucial role in the response of cotton to salt stress.

摘要

背景

脂肪酸羟化酶（FAHs）是一类酶，包括脂肪酸羟化酶、类胡萝卜素羟化酶和甾醇去饱和酶。脂肪酸对植物非常重要。它们是能量储存的主要来源和细胞膜的主要成分。饱和脂肪酸可分为两类：饱和脂肪酸和不饱和脂肪酸。FAHs通过调节脂肪酸代谢途径在增强植物耐盐性方面发挥关键作用，从而提高细胞膜稳定性和抗氧化能力。

结果

在本研究中，我们在四个棉花物种，即陆地棉、达尔文棉、亚洲棉和雷蒙德氏棉中总共鉴定出129个FAH基因家族成员。通过进化分析，FAH基因被分为五个亚组。通过进化树、基因结构和保守基序分析，位于同一亚组的FAH基因呈现出相似的基因结构和保守基序的一致分布。FAH基因家族的染色体定位分析表明它经历了染色体片段重复事件。顺式作用元件分析表明，FAH基因可能参与调节生物和非生物胁迫、植物生长发育、信号通路及其他生理过程。RT-qPCR结果显示，与对照组相比，盐胁迫条件下FAH基因家族成员的表达水平存在显著差异。此外，我们通过VIGS实验成功沉默了Gohir.A03G045300，结果表明沉默植株比对照植株对盐胁迫更敏感。这表明Gohir.A03G045300可能参与棉花对盐胁迫的响应。