Aldana-Mejía Jennyfer A, Ribeiro Victor Pena, Katragunta Kumar, Avula Bharathi, Tatapudi Kiran Kumar, Bastos Jairo Kenupp, Khan Ikhlas A, Meepagala Kumudini, Ross Samir A
National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS 38677, USA.
Agricultural Research Service, Natural Products Utilization Research Unit, U.S. Department of Agriculture, University, MS 38677, USA.
Plants (Basel). 2024 Dec 21;13(24):3576. doi: 10.3390/plants13243576.
Green propolis, particularly from the unique flora of the Brazilian Caatinga biome, has gained significant interest due to its diverse chemical composition and biological activities. This study focuses on the chemical characterization and antimicrobial evaluation of Caatinga green propolis. Twelve compounds were isolated through different chromatographic techniques, including flavanones (naringenin, 7--methyleriodictyol, sakuranetin), flavones (hispidulin, cirsimaritin), flavonols (quercetin, quercetin-3-methyl ether, kaempferol, 6-methoxykaempferol, viscosine, penduletin), and one chalcone (kukulkanin B). Using liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (LC-QToF-MS), a total of 55 compounds excluding reference standards were tentatively identified, which include flavonoids, phenolic acids derivatives, and alkaloids, with flavonols, flavanones, and flavones being predominant. Antimicrobial testing against pathogens revealed that the crude extract exhibited low inhibitory activity, against Gram-positive bacteria, including methicillin-resistant (MRSA) and vancomycin-resistant (VRE) (IC: 148.4 and 120.98 µg/mL, respectively). Although the isolated compounds showed limited individual activity, a fraction containing sakuranetin and penduletin (Fraction 8) exhibited moderated activity against (IC: 47.86 µg/mL), while a fraction containing quercetin and hispidulin showed moderated activity against VRE (IC: 16.99 µg/mL). These findings highlight the potential application of Caatinga green propolis as an antimicrobial agent, particularly against resistant bacterial strains, and underscore the importance of synergistic interactions between compounds in enhancing biological effects.
绿蜂胶，尤其是来自巴西卡廷加生物群落独特植物群的绿蜂胶，因其多样的化学成分和生物活性而备受关注。本研究聚焦于卡廷加绿蜂胶的化学表征和抗菌评估。通过不同的色谱技术分离出了12种化合物，包括黄烷酮（柚皮素、7 - 甲基圣草酚、樱花素）、黄酮（兔儿伞素、 cirsimaritin）、黄酮醇（槲皮素、槲皮素 - 3 - 甲醚、山奈酚、6 - 甲氧基山奈酚、粘菌素、penduletin）以及一种查尔酮（kukulkanin B）。使用液相色谱 - 四极杆飞行时间串联质谱（LC - QToF - MS），初步鉴定出了总共55种不包括参考标准品的化合物，其中包括黄酮类、酚酸衍生物和生物碱，黄酮醇、黄烷酮和黄酮占主导。对病原体的抗菌测试表明，粗提物对革兰氏阳性菌表现出低抑制活性，包括耐甲氧西林金黄色葡萄球菌（MRSA）和耐万古霉素肠球菌（VRE）（IC50分别为148.4和120.98 µg/mL）。尽管分离出的化合物个体活性有限，但含有樱花素和penduletin的馏分（馏分8）对MRSA表现出中等活性（IC50为47.86 µg/mL），而含有槲皮素和兔儿伞素的馏分对VRE表现出中等活性（IC50为16.99 µg/mL）。这些发现突出了卡廷加绿蜂胶作为抗菌剂的潜在应用，特别是对抗耐药菌株，并强调了化合物之间协同相互作用在增强生物效应方面的重要性。
