Cavalcante Giani Maria, Camara Celso Amorim, Silva Eva Monica Sarmento Da, Santos Mariana Silva, Leite Anderson Brandão, Queiroz Aline Cavalcanti, Evelyn Da Silva Amanda, Araújo Morgana Vital, Alexandre-Moreira Magna Suzana, Silva Tania Maria Sarmento
Phytochemical Bioprospecting Laboratory, Chemistry Department, Rural Federal University of Pernambuco, Pernambuco, Brazil.
Zootechnical Collegiate, Federal University of the São Francisco Valley, Pernambuco, Brazil.
Front Pharmacol. 2021 Jul 1;12:702032. doi: 10.3389/fphar.2021.702032. eCollection 2021.
The aim of the current study is to investigate the chemical composition, cytotoxic effect, and leishmanicidal activity of propolis collected in the semi-arid region of Bahia, Brazil. EtOH extract, hexane, EtOAc and MeOH fractions from propolis were analyzed by ultra-performance liquid chromatography coupled with diode array detector and quadrupole time-of-flight mass spectrometry. The identification was based on the exact mass, general fragmentation behaviors and UV absorption of the flavonoids. The cytotoxic effect and leishmanicidal activity of ethanolic extract, hexane, ethyl acetate, and methanolic fractions of propolis were evaluated. Five triterpenes and twenty-four flavonoids were identified. The propolis did not present toxicity to the host cell up to the maximum concentration tested. In addition, all tested samples showed statistically significant activity against promastigotes of and Regarding the activity against amastigote forms of , the hexane fraction, presented statistically significant activity with IC of 1.3 ± 0.1 μg/ml. The results support the idea that propolis can be used for future antileishmania studies.
本研究的目的是调查在巴西巴伊亚半干旱地区采集的蜂胶的化学成分、细胞毒性作用和杀利什曼原虫活性。采用超高效液相色谱联用二极管阵列检测器和四极杆飞行时间质谱对蜂胶的乙醇提取物、己烷、乙酸乙酯和甲醇馏分进行分析。鉴定基于黄酮类化合物的精确质量、一般裂解行为和紫外吸收。评估了蜂胶乙醇提取物、己烷、乙酸乙酯和甲醇馏分的细胞毒性作用和杀利什曼原虫活性。鉴定出5种三萜类化合物和24种黄酮类化合物。在所测试的最高浓度下，蜂胶对宿主细胞没有毒性。此外，所有测试样品对 和 的前鞭毛体均表现出统计学上显著的活性。关于对无鞭毛体形式的活性，己烷馏分表现出统计学上显著的活性，IC为1.3±0.1μg/ml。结果支持蜂胶可用于未来抗利什曼原虫研究的观点。
