Mazur Marek Witold, Grudniak Anna, Szałaj Urszula, Szerszeń Marcin, Mizeracki Jan, Cierech Mariusz, Mierzwińska-Nastalska Elżbieta, Kostrzewa-Janicka Jolanta
Department of Prosthodontics, Medical University of Warsaw, 02-097 Warsaw, Poland.
Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, 02-096 Warsaw, Poland.
Polymers (Basel). 2024 Dec 17;16(24):3512. doi: 10.3390/polym16243512.
Incorporating nanoparticles into denture materials shows promise for the prevention of denture-associated fungal infections. This study investigates the antifungal properties of acrylic modified with microwave-sintered ZnO-Ag nanoparticles. ZnO-Ag nanoparticles (1% and 2.5% wt.) were synthesized via microwave solvothermal synthesis (MSS). Nanoparticles were characterized for phase purity, specific surface area (SSA), density, morphology, and elemental composition. ZnO and ZnO-Ag nanoparticles were added to acrylic material (PMMA) at concentrations of 1% and 2.5% and polymerized. Pure PMMA (control) and obtained PMMA-nanocomposites were cut into homogeneous 10 × 10 mm samples. Antifungal activity of nanoparticles and PMMA-nanocomposites against was tested using minimal inhibitory concentration (MIC) determination, and biofilm formation was assessed using crystal violet staining followed by absorbance measurements. Laboratory tests confirmed phase purity and uniform, spherical particle distribution. MIC results show antifungal activity of 1% Ag nanoparticles and the PMMA-2.5% (ZnO-1% Ag) nanocomposite. PMMA-1% (ZnO-1% Ag) nanocomposite and 1% ZnO-Ag nanoparticles are efficient in preventing biofilm formation. However, ZnO nanoparticles showed antibiofilm activity, and the PMMA-ZnO nanocomposite does not protect against biofilm deposition. Incorporating hybrid ZnO-Ag nanoparticles into PMMA is a promising antibiofilm method, especially with ZnO-1% Ag nanoparticles.
将纳米颗粒掺入义齿材料中有望预防义齿相关真菌感染。本研究调查了用微波烧结的ZnO-Ag纳米颗粒改性的丙烯酸树脂的抗真菌性能。通过微波溶剂热合成（MSS）合成了1%和2.5%（重量）的ZnO-Ag纳米颗粒。对纳米颗粒的相纯度、比表面积（SSA）、密度、形态和元素组成进行了表征。将ZnO和ZnO-Ag纳米颗粒以1%和2.5%的浓度添加到丙烯酸材料（PMMA）中并进行聚合。将纯PMMA（对照）和所得的PMMA纳米复合材料切成均匀的10×10mm样品。使用最小抑菌浓度（MIC）测定法测试纳米颗粒和PMMA纳米复合材料对的抗真菌活性，并使用结晶紫染色后进行吸光度测量来评估生物膜形成。实验室测试证实了相纯度以及颗粒分布均匀且呈球形。MIC结果显示1%Ag纳米颗粒和PMMA-2.5%（ZnO-1%Ag）纳米复合材料具有抗真菌活性。PMMA-1%（ZnO-1%Ag）纳米复合材料和1%ZnO-Ag纳米颗粒在预防生物膜形成方面很有效。然而，ZnO纳米颗粒显示出抗生物膜活性，而PMMA-ZnO纳米复合材料不能防止生物膜沉积。将混合的ZnO-Ag纳米颗粒掺入PMMA是一种有前景的抗生物膜方法，尤其是使用ZnO-1%Ag纳米颗粒时。
