Cierech Mariusz, Wojnarowicz Jacek, Szmigiel Dariusz, Bączkowski Bohdan, Grudniak Anna Maria, Wolska Krystyna Izabela, Łojkowski Witold, Mierzwińska-Nastalska Elżbieta
Department of Prosthetic Dentistry, Medical University of Warsaw, Poland.
Institute of High Pressure Physic, Polish Academy of Sciences, Warsaw, Poland.
Acta Bioeng Biomech. 2016;18(2):31-41.
The aim of the paper was to investigate the antifungal activity of zinc oxide nanoparticles (ZnONPs) against Candida albicans. Some attempts have been made to find out the best way to introduce ZnONPs into polymethyl methacrylate (PMMA) resin material and to determine some parameters of a newly formed composite.
Zinc oxide nanoparticles were manufactured and their basic physical parameters were determined (average particle size, density, specific surface area). Minimal inhibitory concentration (MIC) of ZnONPs was determined for the Candida albicans standard strain. The average size of ZnO conglomerates in the monomer solution of PMMA resin was measured using a dynamic light scattering instrument. PMMA resin samples with incorporated ZnONPs were produced. The morphology of nanopowder and the newly formed composite was examined under a scanning electron microscope (SEM). In addition, the roughness parameter of PMMA resin material was investigated before and after ZnONPs modification.
Nanopowder with the average particle size of 30 nm, density of 5.24 g/cm3 and surface area of 39 m2/g was obtained. MIC was determined at the level of 0.75 mg/mL. The average size of ZnO conglomerates in the monomer solution of acrylic resin dropped by 11 times after ultrasound activation. SEM examination of a newly formed composite showed a successful introduction of ZnONPs confirmed by the energy dispersive X-ray spectroscopy (EDS) analysis. There were no statistically significant differences in the biomaterial roughness before and after the modification of ZnONPs.
Zinc oxide nanoparticles were successfully incorporated into acrylic resin used for the production of denture bases. The presence of nanoparticles with sizes below 100 nm was confirmed. Nevertheless a newly created composite needs to be further investigated to improve its homogeneity, and to check its microbiological properties, strength and biocompatibility prior to its possible clinical use.
本文旨在研究氧化锌纳米颗粒（ZnONPs）对白色念珠菌的抗真菌活性。已进行了一些尝试，以找出将ZnONPs引入聚甲基丙烯酸甲酯（PMMA）树脂材料的最佳方法，并确定新形成复合材料的一些参数。
制备氧化锌纳米颗粒并测定其基本物理参数（平均粒径、密度、比表面积）。测定ZnONPs对白色念珠菌标准菌株的最小抑菌浓度（MIC）。使用动态光散射仪测量PMMA树脂单体溶液中ZnO聚集体的平均尺寸。制备了含有ZnONPs的PMMA树脂样品。在扫描电子显微镜（SEM）下检查纳米粉末和新形成复合材料的形态。此外，研究了ZnONPs改性前后PMMA树脂材料的粗糙度参数。
获得了平均粒径为30nm、密度为5.24g/cm³、表面积为39m²/g的纳米粉末。MIC测定为0.75mg/mL。超声激活后，丙烯酸树脂单体溶液中ZnO聚集体的平均尺寸下降了11倍。对新形成复合材料的SEM检查显示，能量色散X射线光谱（EDS）分析证实ZnONPs成功引入。ZnONPs改性前后生物材料粗糙度无统计学显著差异。
氧化锌纳米颗粒已成功掺入用于制作义齿基托的丙烯酸树脂中。证实了存在尺寸小于100nm的纳米颗粒。然而，新创建的复合材料需要进一步研究，以改善其均匀性，并在其可能临床应用之前检查其微生物学特性、强度和生物相容性。
