De Luca Maria, Casula Luca, Tuberoso Carlo Ignazio Giovanni, Pons Ramon, Morán Maria Del Carmen, García María Teresa, Martelli Giuseppe, Vassallo Antonio, Caddeo Carla
Department of Science, University of Basilicata, Viale dell'Ateneo Lucano 10, 85100 Potenza, Italy.
KAMABIO Srl, Via Al Boschetto 4/B, 39100 Bolzano, Italy.
Pharmaceutics. 2024 Nov 23;16(12):1507. doi: 10.3390/pharmaceutics16121507.
: Horseradish ( L.) roots-largely used in traditional medicine for their multiple therapeutic effects-are a rich source of health-promoting phytochemicals. However, their efficacy can be compromised by low chemical stability and poor bioavailability. Incorporation into phospholipid vesicles is often proposed to tackle this problem. : In this study, a hydroalcoholic extract was produced from horseradish roots. The extract was characterized by UPLC-MS and HPLC-PDA and formulated in conventional liposomes and Penetration Enhancer-containing Vesicles (PEVs) for skin application. : The obtained nanovesicles were small in size (<100 nm), negatively charged, uni/bilamellar, and with high values of entrapment efficiency (>85%) for the flavonoids identified in the extract. Both the free and the nanoformulated extract showed optimal biocompatibility, measured as the absence of hemolysis of erythrocytes and absence of cytotoxicity in skin cell lines. Furthermore, the nanoformulations displayed antioxidant activity in vitro. : The proposed nananoformulations could be exploited to counteract oxidative stress involved in the pathogenesis and progression of numerous skin disorders.
辣根（L.）根因其多种治疗作用而在传统医学中广泛使用，是促进健康的植物化学物质的丰富来源。然而，其功效可能会因化学稳定性低和生物利用度差而受到影响。人们通常建议将其包封于磷脂囊泡中来解决这个问题。
在本研究中，制备了辣根根的水醇提取物。该提取物通过超高效液相色谱-质谱联用仪（UPLC-MS）和高效液相色谱-光电二极管阵列检测器（HPLC-PDA）进行表征，并制成传统脂质体和含渗透促进剂囊泡（PEV）用于皮肤给药。
所获得的纳米囊泡尺寸小（<100 nm），带负电荷，单/双层，对提取物中鉴定出的黄酮类化合物具有高包封率（>85%）。游离提取物和纳米制剂均表现出最佳的生物相容性，这通过红细胞不溶血和皮肤细胞系无细胞毒性来衡量。此外，纳米制剂在体外具有抗氧化活性。
所提出的纳米制剂可用于对抗多种皮肤疾病发病机制和进展中涉及的氧化应激。
