白藜芦醇负载纳米颗粒对体外肺肿瘤球体形成和生长的防癌作用评估。

Evaluation of the Cancer-Preventive Effect of Resveratrol-Loaded Nanoparticles on the Formation and Growth of In Vitro Lung Tumor Spheroids.

作者信息

Torrico Guzmán Elisa A, Gravely Mitchell, Meenach Samantha A

机构信息

Department of Chemical Engineering, College of Engineering, University of Rhode Island, Kingston, RI 02881, USA.

Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI 02881, USA.

出版信息

Pharmaceutics. 2024 Dec 12;16(12):1588. doi: 10.3390/pharmaceutics16121588.

DOI:10.3390/pharmaceutics16121588

PMID:39771566

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11677875/

Abstract

BACKGROUND

Resveratrol (RSV) is a natural polyphenol that offers antioxidant, anti-inflammatory, and chemopreventive benefits. This project determined the ability of RSV-loaded nanoparticles (NP) to inhibit the growth of lung tumor spheroids in vitro.

METHODS

RSV was encapsulated in NP comprised of the biodegradable polymer, acetalated dextran. A549 lung cancer cells in two-dimensional and three-dimensional cell culture models were exposed to free RSV and RSV NP to evaluate their effect on cell proliferation and spheroid formation and growth. For prevention studies, spheroids were exposed to free RSV and RSV NP on day 0, and for treatment studies, spheroids were dosed with the same formulations on day 5 after the spheroids had fully formed.

RESULTS

The resulting RSV NP were 200 nm in diameter with neutral surface charge and exhibited the ability to control the release of RSV in vitro based on environmental pH. In comparison to free RSV, the RSV NP exerted a greater inhibitory effect on the proliferation and growth of cancer cells and spheroids.

CONCLUSIONS

RSV NP have the potential to be used as a chemopreventive agent for lung cancer.

摘要

背景

白藜芦醇（RSV）是一种天然多酚，具有抗氧化、抗炎和化学预防作用。本项目测定了负载白藜芦醇的纳米颗粒（NP）在体外抑制肺肿瘤球体生长的能力。

方法

将白藜芦醇包裹于由可生物降解聚合物乙酰化葡聚糖组成的纳米颗粒中。在二维和三维细胞培养模型中，将A549肺癌细胞暴露于游离白藜芦醇和白藜芦醇纳米颗粒中，以评估它们对细胞增殖以及球体形成和生长的影响。对于预防研究，在第0天将球体暴露于游离白藜芦醇和白藜芦醇纳米颗粒；对于治疗研究，在球体完全形成后的第5天用相同制剂给药。