Vorovitch Mikhail F, Tuchynskaya Ksenia K, Kruglov Yuriy A, Peunkov Nikita S, Mostipanova Guzal F, Kholodilov Ivan S, Ivanova Alla L, Fedina Maria P, Gmyl Larissa V, Morozkin Evgeny S, Roev German V, Karan Lyudmila S, Karganova Galina G
Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis), Moscow 108819, Russia.
Institute of Translational Medicine and Biotechnology, Sechenov First Moscow State Medical University, Moscow 119991, Russia.
Vaccines (Basel). 2024 Dec 12;12(12):1398. doi: 10.3390/vaccines12121398.
: West Nile virus (WNV) is a rapidly growing problem worldwide. The lack of emergency treatment and a safe licensed vaccine against WNV allows the virus to cause sporadic outbreaks of human disease, including fatal cases. Formalin-inactivated vaccines have been used for a long time and have been shown to be very safe and effective, especially in susceptible populations. By adapting tick-borne encephalitis vaccine production technology, we produced a laboratory-inactivated vaccine against WNV based on the strain SHUA, isolated from humans with a lethal WNV infection in the year 2021. : The potential vaccine was tested for safety in vitro and in vivo in outbred SHK mice of different ages, including PCR analysis of the brains of these mice to test for the absence of viral RNA after intracerebral injection. The inactivated whole-virion laboratory vaccine showed 100% seroconversion and immunogenicity against WNV strain SHUA-1, isolated from a lethal human case, and provided the mice with 100% protection from disease and death.
西尼罗河病毒（WNV）在全球范围内是一个迅速发展的问题。缺乏针对WNV的紧急治疗方法和安全的许可疫苗，使得该病毒能够引发人类疾病的零星爆发，包括致命病例。福尔马林灭活疫苗已经使用了很长时间，并且已被证明非常安全有效，尤其是在易感人群中。通过采用蜱传脑炎疫苗生产技术，我们基于2021年从感染致命WNV的人类中分离出的SHUA毒株，生产了一种针对WNV的实验室灭活疫苗。对该潜在疫苗在不同年龄的远交SHK小鼠体内和体外进行了安全性测试，包括对这些小鼠的大脑进行PCR分析，以检测脑内注射后是否存在病毒RNA。这种灭活的全病毒实验室疫苗对从致命人类病例中分离出的WNV毒株SHUA - 1显示出100%的血清转化和免疫原性，并为小鼠提供了100%的疾病和死亡保护。
