Tabler Caroline O, Tilton John C
Center for Proteomics and Bioinformatics, Department of Nutrition, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA.
Viruses. 2024 Nov 25;16(12):1826. doi: 10.3390/v16121826.
The HIV-1 protease is a critical enzyme for viral replication. Because protease activity is necessary to generate mature infectious virions, it is a primary target of antiretroviral treatment. Here, we provide an overview of the mechanisms regulating protease activation and the methods available to assess protease activity. Finally, we will highlight some of the key discoveries regarding the kinetics of protease activation from the last decade, including how the manipulation of activation kinetics may provide novel HIV-1 treatment strategies.
HIV-1蛋白酶是病毒复制的关键酶。由于蛋白酶活性对于产生成熟的感染性病毒粒子是必需的，因此它是抗逆转录病毒治疗的主要靶点。在此，我们概述调节蛋白酶激活的机制以及可用于评估蛋白酶活性的方法。最后，我们将重点介绍过去十年中关于蛋白酶激活动力学的一些关键发现，包括激活动力学的操纵如何可能提供新的HIV-1治疗策略。
