通过流病毒仪分析个体病毒颗粒。

Analysis of Individual Viral Particles by Flow Virometry.

机构信息

Center for Proteomics and Bioinformatics, Department of Nutrition, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA.

出版信息

Viruses. 2024 May 18;16(5):802. doi: 10.3390/v16050802.

DOI:10.3390/v16050802

PMID:38793683

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11125929/

Abstract

This review focuses on the emerging field of flow virometry, the study and characterization of individual viral particles using flow cytometry instruments and protocols optimized for the detection of nanoscale events. Flow virometry faces considerable technical challenges including minimal light scattering by small viruses that complicates detection, coincidental detection of multiple small particles due to their high concentrations, and challenges with sample preparation including the inability to easily "wash" samples to remove unbound fluorescent antibodies. We will discuss how the field has overcome these challenges to reveal novel insights into viral biology.

摘要

本综述聚焦于新兴的流场病毒学领域，该领域使用经优化用于检测纳尺度事件的流式细胞仪仪器和方案来研究和表征单个病毒颗粒。流场病毒学面临着相当大的技术挑战，包括小病毒的光散射极小，这使得检测变得复杂，由于高浓度，多个小颗粒的偶然检测，以及样品制备方面的挑战，包括难以“清洗”样品以去除未结合的荧光抗体。我们将讨论该领域如何克服这些挑战，以揭示病毒生物学的新见解。