Wang Chen, Tang Yao, Zhou Changmei, Li Shanshan, Chen Jianping, Sun Zongtao
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
Viruses. 2024 Nov 29;16(12):1866. doi: 10.3390/v16121866.
Rice is a crucial staple food for over half the global population, and viral infections pose significant threats to rice yields. This study focuses on the Rice Stripe Virus (RSV), which is known to drastically reduce rice productivity. We employed RNA-seq and ribosome profiling to analyze the transcriptional and translational responses of RSV-infected rice seedlings. Our results reveal that translational reprogramming is a critical aspect of the plant's defense mechanism, operating independently of transcriptional changes. Notably, less than half of the differentially expressed genes showed concordance between transcription and translation. Furthermore, RSV infection led to significant alterations in translational efficiency for numerous genes, suggesting that the virus selectively manipulates translation to enhance its pathogenicity. Our findings underscore the necessity of examining both transcriptional and translational landscapes to fully understand plant responses to viral infections.
水稻是全球一半以上人口的关键主食，病毒感染对水稻产量构成重大威胁。本研究聚焦于水稻条纹病毒（RSV），已知该病毒会大幅降低水稻生产力。我们采用RNA测序和核糖体分析来分析受RSV感染的水稻幼苗的转录和翻译反应。我们的结果表明，翻译重编程是植物防御机制的一个关键方面，其运作独立于转录变化。值得注意的是，不到一半的差异表达基因在转录和翻译之间表现出一致性。此外，RSV感染导致许多基因的翻译效率发生显著变化，这表明该病毒选择性地操纵翻译以增强其致病性。我们的研究结果强调了同时研究转录和翻译情况以全面了解植物对病毒感染反应的必要性。
