Yum Su Jin, Yu Seon Yeong, Kim Seung Min, Jeong Hee Gon
Department of Food Science and Technology, College of Agriculture and Life Sciences, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, South Korea.
Division of Human Ecology, Korea National Open University, 86 Daehak-ro, Jongno-gu, Seoul 03087, South Korea.
J Agric Food Chem. 2025 Jan 22;73(3):2156-2166. doi: 10.1021/acs.jafc.4c11161. Epub 2025 Jan 8.
Antimicrobial resistance (AMR) poses a critical global public health challenge. This study investigates the microbiome of var. (kale) to evaluate the role of food production systems, particularly plant-derived foods, in AMR dissemination. Using 16S rRNA gene sequencing and metagenomic shotgun sequencing, we analyzed microbial diversity and antimicrobial resistance genes (ARGs) in kale samples. Results showed significant regional differences in microbiota composition and ARG distribution, with traditional fertilizer use linked to higher ARG prevalence in coliform bacteria compared to farms using other fertilization methods. Additionally, we confirmed ARG transfer potential by within coliform populations. Storage conditions notably affected microbial dynamics, with higher temperatures promoting growth in washed samples. These findings revealed the importance of AMR research in plant-derived foods and highlight the need for improved agricultural practices to mitigate the risks associated with high ARG abundance in coliform bacteria.
抗菌药物耐药性（AMR）对全球公共卫生构成了严峻挑战。本研究调查了羽衣甘蓝变种的微生物组，以评估食品生产系统，特别是植物源性食品在AMR传播中的作用。我们使用16S rRNA基因测序和宏基因组鸟枪法测序，分析了羽衣甘蓝样本中的微生物多样性和抗菌药物耐药基因（ARGs）。结果显示，微生物群组成和ARGs分布存在显著的区域差异，与使用其他施肥方法的农场相比，传统肥料的使用与大肠菌群中更高的ARGs流行率相关。此外，我们通过大肠菌群群体内的[具体内容缺失]证实了ARGs的转移潜力。储存条件显著影响微生物动态，较高的温度促进了洗涤样本中的[具体内容缺失]生长。这些发现揭示了植物源性食品中AMR研究的重要性，并强调了改进农业实践以降低与大肠菌群中高ARGs丰度相关风险的必要性。
