Hou Tianfei, Su Wen, Chacon Aaron N, Lin An-Hsuan, Guo Zhenheng, Gong Ming C
Department of Physiology, College of Medicine, University of Kentucky, Lexington, Kentucky.
Department of Pharmacology and Nutritional Sciences, College of Medicine, University of Kentucky, Lexington, Kentucky.
J Biol Rhythms. 2025 Feb;40(1):76-90. doi: 10.1177/07487304241302510. Epub 2025 Jan 8.
Cardiovascular health requires the orchestration of the daily rhythm of blood pressure (BP), which responds to changes in light exposure and dietary patterns. Whether rhythmic light and feeding can modulate daily BP rhythm directly or via modulating intrinsic core clock gene is unknown. Using inducible global knockout mice (iBmal1KO), we explored the impact of rhythmic light, rhythmic feeding, or their combination on various physiological parameters. Daily rhythms of BP, heart rate, and locomotor activity were monitored via radiotelemetry, while food intake patterns were tracked using the BioDAQ system. Respiratory exchange ratio (RER) and energy expenditure (EE) were assessed through indirect calorimetry. In addition, spectrum analysis was employed to analyze spontaneous baroreflex sensitivity and heart rate variability, and urinary norepinephrine excretion was quantified using high-performance liquid chromatography (HPLC). Neither rhythmic feeding nor rhythmic light alone was sufficient to reinstate the daily BP rhythm in arrhythmic iBmal1KO mice. However, combining the light and feeding cues in synchrony partially restored the daily BP rhythm. Interestingly, rhythmic feeding alone robustly reinstated RER and EE rhythms, even without rhythmic light. Similar to BP, the partial reinstatement of the daily rhythms in heart rate and locomotor activity was observed only when rhythmic light and feeding were applied in tandem. Rhythmic light by itself did not restore the light-dark phase difference in baroreflex sensitivity, urinary norepinephrine excretion, or the daily rhythm of heart rate variability. However, rhythmic feeding, alone or in combination with rhythmic light, successfully reinstated the light-dark phase differences in these parameters. In the absence of , the synergy between rhythmic light and feeding can partially restore daily BP rhythm.
心血管健康需要协调血压的日常节律，血压会对光照和饮食模式的变化做出反应。有节奏的光照和进食是否能直接调节日常血压节律，或通过调节内在核心时钟基因来调节，目前尚不清楚。我们使用诱导型全球基因敲除小鼠（iBmal1KO），探讨了有节奏的光照、有节奏的进食或它们的组合对各种生理参数的影响。通过无线电遥测监测血压、心率和运动活动的日常节律，同时使用BioDAQ系统跟踪食物摄入模式。通过间接量热法评估呼吸交换率（RER）和能量消耗（EE）。此外，采用频谱分析来分析自发压力反射敏感性和心率变异性，并使用高效液相色谱法（HPLC）对尿去甲肾上腺素排泄进行定量。单独的有节奏进食或有节奏光照都不足以恢复心律失常的iBmal1KO小鼠的日常血压节律。然而，将光照和进食线索同步结合可部分恢复日常血压节律。有趣的是，仅节律性进食就能有力地恢复RER和EE节律，即使没有节律性光照。与血压类似，只有当节律性光照和进食同时应用时，才会观察到心率和运动活动的日常节律部分恢复。单独的节律性光照并不能恢复压力反射敏感性、尿去甲肾上腺素排泄或心率变异性日常节律中的明暗相位差异。然而，节律性进食单独或与节律性光照结合，成功恢复了这些参数中的明暗相位差异。在没有（此处原文缺失相关内容）的情况下，节律性光照和进食之间的协同作用可部分恢复日常血压节律。
