主客体识别介导的脱氧核酶活性的可逆正交调控。

Host-guest recognition-mediated reversible and orthogonal regulation of DNAzyme activity.

作者信息

Xiao Lin, Zhu Haitao, Gao Yanmei, Xu Liang

机构信息

School of Laboratory Medicine, Department of Clinical Laboratory, Affiliated Hospital of North Sichuan Medical College & Translational Medicine Research Center, North Sichuan Medical College, Nanchong 637000, China.

MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-Sen University, Guangzhou 510275, China.

出版信息

Chem Commun (Camb). 2025 Jan 23;61(9):1890-1893. doi: 10.1039/d4cc05330c.

DOI:10.1039/d4cc05330c

PMID:39775627

Abstract

The host-guest recognition system is incorporated into the core region of the 10-23 DNAzyme for precise regulation of its functionality. Biochemical experiments demonstrate reversible and orthogonal control of the DNAzyme function using cucurbit(7)uril and its competitive guests. Furthermore, cellular experiments indicate the gene expression can be effectively manipulated through this ligand-controllable DNAzyme.

摘要

主客体识别系统被整合到10-23脱氧核酶的核心区域，以精确调控其功能。生化实验证明，使用葫芦脲(7)及其竞争性客体可对脱氧核酶功能进行可逆且正交的控制。此外，细胞实验表明，通过这种配体可控的脱氧核酶可以有效地操纵基因表达。