Morris J S, Meakins J L, Christou N V
Arch Surg. 1985 Feb;120(2):205-9. doi: 10.1001/archsurg.1985.01390260065009.
We examined the interrelationships between leukocyte adherence (ADH), polymorphonuclear neutrophil (PMN) chemotaxis (CTX), and PMN delivery to an inflammatory focus in hospitalized patients. Patients included 25 men and 17 women, with a mean age of 62.8 years, who were admitted for major elective surgery. The patients were studied preoperatively and on the second and seventh postoperative days. Leukocyte adherence increased on the second postoperative day (82.3%, P less than .05) and remained elevated on the seventh postoperative day (81.6%). Polymorphonuclear neutrophil chemotaxis decreased significantly on the second postoperative day (3.4 to 2.9 cm, P less than .05), but returned to normal by the seventh postoperative day. The PMN delivery to skin-window chambers decreased markedly on the second postoperative day (1.28 million cells, P less than .05) with a further decrease on the seventh postoperative day (0.82 million cells). There was no correlation between in vitro PMN CTX and in vivo cell delivery. We conclude that major surgery leads to increased ADH, decreased PMN CTX, and diminished PMN delivery to areas of inflammation. However, a single measure of PMN function such as in vitro PMN CTX does not, alone, reflect in vivo PMN delivery to areas of inflammation.
我们研究了住院患者白细胞黏附（ADH）、多形核中性粒细胞（PMN）趋化性（CTX）以及PMN向炎症部位输送之间的相互关系。患者包括25名男性和17名女性，平均年龄62.8岁，因择期大手术入院。在术前、术后第2天和第7天对患者进行研究。术后第2天白细胞黏附增加（82.3%，P<0.05），术后第7天仍保持升高（81.6%）。术后第2天多形核中性粒细胞趋化性显著降低（从3.4降至2.9厘米，P<0.05），但术后第7天恢复正常。术后第2天PMN向皮肤窗小室的输送显著减少（128万个细胞，P<0.05），术后第7天进一步减少（82万个细胞）。体外PMN CTX与体内细胞输送之间无相关性。我们得出结论，大手术导致ADH增加、PMN CTX降低以及PMN向炎症区域的输送减少。然而，单一的PMN功能指标，如体外PMN CTX，单独并不能反映体内PMN向炎症区域的输送情况。
