Molatefi Rasol, Fouladi Nasrin, Asghariazar Vahid, Samemaleki Sahar, Golizadeh Majid, Khoshlega Sepehr, Safarzadeh Elham
Cancer Immunology and Immunotherapy Research Center, Ardabil University of Medical Sciences, Ardabil, Iran.
Department of Pediatrics, Iran3. School of Medicine and Allied Medical Sciences, Bo-Ali Children's Hospital of Ardabil University of Medical Sciences, Ardabil University of Medical Sciences, Ardabil, Iran.
Arch Dermatol Res. 2025 Jan 7;317(1):182. doi: 10.1007/s00403-024-03709-3.
Atopic dermatitis (AD) is a chronic inflammatory disease characterized by increased skin sensitivity to environmental elements, mediated by CD4 T helper cells (Th2). Interleukin-33 (IL-33) plays a critical role in exacerbating symptoms in inflamed tissues. Conversely, vitamin D has been shown to induce antimicrobial peptides and suppress the inflammatory response. Therefore, the purpose of this study was to investigate the correlation between serum levels of vitamin D and IL-33 in children with Atopic dermatitis.
Blood samples were collected from 51 patients with Atopic dermatitis and 20 healthy control cases. After counting the blood cells, serum was isolated by centrifugation, and the levels of IL-33, Immunoglobulin E (IgE), and vitamin D were measured using ELISA. Statistical analysis of the collected data was performed using SPSS version 26 software.
Our findings revealed significant differences in the levels of IL- 33 (p value < 0.001), IgE (p value 0.005), and blood cell parameters including Hemoglobin (HGB) (p value < 0.001), Hematocrit (HCT) (p value < 0.001), Mean Corpuscular Hemoglobin (MCH) (p value 0.001), Mean Corpuscular Hemoglobin Concentration (MCHC) (p value < 0.001), lymphocyte count (p value 0.02), and monocyte count (p value < 0.001) in AD patients compared to the control group. Additionally, there was a significant correlation between IL-33, and vitamin D serum levels in AD patients (p value 0.03).
The current study has revealed a significant difference in the serum levels of IL-33 and IgE between AD patients and healthy individuals. This suggests a potential role for these variables in the pathophysiology of AD disease.
特应性皮炎（AD）是一种慢性炎症性疾病，其特征是皮肤对环境因素的敏感性增加，由CD4辅助性T细胞（Th2）介导。白细胞介素-33（IL-33）在加剧炎症组织中的症状方面起关键作用。相反，维生素D已被证明可诱导抗菌肽并抑制炎症反应。因此，本研究的目的是调查特应性皮炎患儿血清维生素D水平与IL-33之间的相关性。
收集51例特应性皮炎患者和20例健康对照者的血样。血细胞计数后，通过离心分离血清，并使用酶联免疫吸附测定法（ELISA）测量IL-33、免疫球蛋白E（IgE）和维生素D的水平。使用SPSS 26版软件对收集的数据进行统计分析。
我们的研究结果显示，与对照组相比，AD患者的IL-33水平（p值<0.001）、IgE水平（p值0.005）以及血细胞参数，包括血红蛋白（HGB）（p值<0.001）、血细胞比容（HCT）（p值<0.001）、平均红细胞血红蛋白含量（MCH）（p值0.001）、平均红细胞血红蛋白浓度（MCHC）（p值<0.001）、淋巴细胞计数（p值0.02）和单核细胞计数（p值<0.001）存在显著差异。此外，AD患者的IL-33与血清维生素D水平之间存在显著相关性（p值0.03）。
目前的研究表明，AD患者与健康个体之间的血清IL-33和IgE水平存在显著差异。这表明这些变量在AD疾病的病理生理学中可能发挥作用。
