von Maltzahn Julia
Brandenburg Technische Universität Cottbus-Senftenberg, Faculty of Health Sciences, Senftenberg, Germany.
Brandenburg Technische Universität Cottbus-Senftenberg, Faculty of Environment and Natural Sciences, Senftenberg, Germany.
Methods Mol Biol. 2025;2960:9-18. doi: 10.1007/7651_2024_589.
Muscle stem cells (MuSCs) lose a large proportion of their characteristics when removed from their niche, hampering the analysis of muscle stem cell functionality. However, the isolation and culture of single floating myofibers with their adjacent muscle stem cells allow the short-term culture and manipulation of muscle stem cells in conditions as close as possible to the endogenous niche. Here, the isolation, culture and transfection with siRNA of muscle stem cells on their adjacent myofibers from young as well as old mice are described.
肌肉干细胞（MuSCs）从其生态位中移除后会丧失大部分特性，这阻碍了对肌肉干细胞功能的分析。然而，分离并培养带有相邻肌肉干细胞的单个漂浮肌纤维，能够在尽可能接近内源性生态位的条件下对肌肉干细胞进行短期培养和操作。在此，我们描述了从小鼠和老年小鼠的相邻肌纤维中分离、培养肌肉干细胞并进行siRNA转染的方法。
