Tan Ying, Lin Xiaojun, Huang Lixing, Yan Qingpi, Wang Jiaen, Weng Qibiao, Zhengzhang Yuwei, Chen Yiran, Ma Ying, Zheng Jiang
State Key Laboratory of Mariculture Breeding, Engineering Research Centre of the Modern Technology for Eel Industry, Ministry of Education, Fisheries College of Jimei University, Xiamen, China.
National Research and Development Center for Eel Processing Technology, Key Laboratory of Eel Aquaculture and Processing of Fujian Province, Fujian Provincial Engineering Research Center for Eel Processing Enterprise, Changle Juquan Food Co. Ltd., Fuzhou, China.
Front Vet Sci. 2024 Dec 24;11:1511234. doi: 10.3389/fvets.2024.1511234. eCollection 2024.
is a common bacterial pathogen in aquaculture, often leading to visceral white spot disease in large yellow croakers (). Previous studies have found that certain aptamers show an efficient antibacterial effect against this pathogen. In this study, we analyzed the transcriptome of to get insights into the antibacterial and inhibitions mechanisms following exposure to the aptamer B4. The results showed seven differentially expressed genes (DEGs) associated with the antibacterial effect of the aptamer, namely gene encoding aldehyde dehydrogenase, the gene of phenylacetyl coenzyme A cyclooxygenase, the gene of ABC transporter proteins, two transposase genes with different positions but identical sequences involved in cutting and splicing DNA sequences, and two hypothetical protein genes with unknown functions. Gene Ontology (GO) analysis showed that the DEGs were mainly involved in DNA-mediated translocation, phenylacetic acid catabolism, growth hormone catabolism, polyamine transporter ATPase activity, betaine aldehyde dehydrogenase activity, ABC transporter protein complex, and other related pathways. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the metabolic pathway of niacin and niacinamide mediated through the gene was the most significant and relevant, followed by the metabolism of phenylalanine, alanine, aspartic acid and glutamic acid. Real-time quantitative PCR validation showed that the changes in the DEGs were consistent with the transcriptome analysis. These results suggest that the antibacterial aptamer B4 may inhibit by blocking the synthesis of essential nucleic acids and proteins through the modulation of these DEGs and inhibiting their metabolic pathways.
是水产养殖中常见的细菌病原体，常导致大黄鱼出现内脏白点病（）。先前的研究发现，某些适体对这种病原体显示出有效的抗菌作用。在本研究中，我们分析了的转录组，以深入了解暴露于适体B4后的抗菌和抑制机制。结果显示有七个与适体抗菌作用相关的差异表达基因（DEG），即编码醛脱氢酶的基因、苯乙酰辅酶A环氧化酶的基因、ABC转运蛋白的基因、两个位置不同但序列相同的参与切割和拼接DNA序列的转座酶基因，以及两个功能未知的假设蛋白基因。基因本体论（GO）分析表明，DEG主要参与DNA介导的易位、苯乙酸分解代谢、生长激素分解代谢、多胺转运ATP酶活性、甜菜碱醛脱氢酶活性、ABC转运蛋白复合体及其他相关途径。京都基因与基因组百科全书（KEGG）分析表明，通过基因介导的烟酸和烟酰胺代谢途径最为显著且相关，其次是苯丙氨酸、丙氨酸、天冬氨酸和谷氨酸的代谢。实时定量PCR验证表明，DEG的变化与转录组分析一致。这些结果表明，抗菌适体B4可能通过调节这些DEG并抑制其代谢途径来阻断必需核酸和蛋白质的合成，从而抑制。
