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拟南芥再生根中的间歇性细胞分裂动态揭示了复杂的长距离相互作用。

Intermittent cell division dynamics in regenerating Arabidopsis roots reveals complex long-range interactions.

作者信息

Fallesen T, Amarteifio S, Pruessner G, Jensen H J, Sena G

机构信息

Department of Life Sciences, Imperial College London, London, UK.

Crick Advanced Light Microscopy, Francis Crick Institute, London, UK.

出版信息

Quant Plant Biol. 2024 Sep 4;5:e7. doi: 10.1017/qpb.2024.7. eCollection 2024.

Abstract

In this work, we present a quantitative comparison of the cell division dynamics between populations of intact and regenerating root tips in the plant model system To achieve the required temporal resolution and to sustain it for the duration of the regeneration process, we adopted a live imaging system based on light-sheet fluorescence microscopy, previously developed in the laboratory. We offer a straightforward quantitative analysis of the temporal and spatial patterns of cell division events showing a statistically significant difference in the frequency of mitotic events and spatial separation of mitotic event clusters between intact and regenerating roots.

摘要

在这项工作中,我们在植物模型系统中对完整根尖群体和再生根尖群体之间的细胞分裂动力学进行了定量比较。为了实现所需的时间分辨率并在再生过程持续期间保持该分辨率,我们采用了基于光片荧光显微镜的实时成像系统,该系统是实验室之前开发的。我们对细胞分裂事件的时间和空间模式进行了直接的定量分析,结果表明完整根尖和再生根尖在有丝分裂事件频率以及有丝分裂事件簇的空间分离方面存在统计学上的显著差异。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2a/11706681/69794073ef94/S2632882824000079_figAb.jpg

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