• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

拟南芥再生根中的间歇性细胞分裂动态揭示了复杂的长距离相互作用。

Intermittent cell division dynamics in regenerating Arabidopsis roots reveals complex long-range interactions.

作者信息

Fallesen T, Amarteifio S, Pruessner G, Jensen H J, Sena G

机构信息

Department of Life Sciences, Imperial College London, London, UK.

Crick Advanced Light Microscopy, Francis Crick Institute, London, UK.

出版信息

Quant Plant Biol. 2024 Sep 4;5:e7. doi: 10.1017/qpb.2024.7. eCollection 2024.

DOI:10.1017/qpb.2024.7
PMID:39777033
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11706681/
Abstract

In this work, we present a quantitative comparison of the cell division dynamics between populations of intact and regenerating root tips in the plant model system To achieve the required temporal resolution and to sustain it for the duration of the regeneration process, we adopted a live imaging system based on light-sheet fluorescence microscopy, previously developed in the laboratory. We offer a straightforward quantitative analysis of the temporal and spatial patterns of cell division events showing a statistically significant difference in the frequency of mitotic events and spatial separation of mitotic event clusters between intact and regenerating roots.

摘要

在这项工作中，我们在植物模型系统中对完整根尖群体和再生根尖群体之间的细胞分裂动力学进行了定量比较。为了实现所需的时间分辨率并在再生过程持续期间保持该分辨率，我们采用了基于光片荧光显微镜的实时成像系统，该系统是实验室之前开发的。我们对细胞分裂事件的时间和空间模式进行了直接的定量分析，结果表明完整根尖和再生根尖在有丝分裂事件频率以及有丝分裂事件簇的空间分离方面存在统计学上的显著差异。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2a/11706681/c74fae8501a1/S2632882824000079_fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2a/11706681/69794073ef94/S2632882824000079_figAb.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2a/11706681/f3d26c01fcd4/S2632882824000079_fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2a/11706681/999af7dca0f3/S2632882824000079_fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2a/11706681/69c58c41fb57/S2632882824000079_fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2a/11706681/c41e644c84a2/S2632882824000079_fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2a/11706681/9784e1a54c83/S2632882824000079_fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2a/11706681/c74fae8501a1/S2632882824000079_fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2a/11706681/69794073ef94/S2632882824000079_figAb.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2a/11706681/f3d26c01fcd4/S2632882824000079_fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2a/11706681/999af7dca0f3/S2632882824000079_fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2a/11706681/69c58c41fb57/S2632882824000079_fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2a/11706681/c41e644c84a2/S2632882824000079_fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2a/11706681/9784e1a54c83/S2632882824000079_fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2a/11706681/c74fae8501a1/S2632882824000079_fig6.jpg

相似文献

1
Intermittent cell division dynamics in regenerating Arabidopsis roots reveals complex long-range interactions.拟南芥再生根中的间歇性细胞分裂动态揭示了复杂的长距离相互作用。
Quant Plant Biol. 2024 Sep 4;5:e7. doi: 10.1017/qpb.2024.7. eCollection 2024.
2
High-resolution live imaging of plant growth in near physiological bright conditions using light sheet fluorescence microscopy.使用光片荧光显微镜在近生理明亮条件下对植物生长进行高分辨率实时成像。
Plant J. 2011 Oct;68(2):377-85. doi: 10.1111/j.1365-313X.2011.04692.x. Epub 2011 Aug 4.
3
Spatiotemporal Pattern of Ectopic Cell Divisions Contribute to Mis-Shaped Phenotype of Primary and Lateral Roots of Mutant.异位细胞分裂的时空模式导致突变体主根和侧根的畸形表型。
Front Plant Sci. 2020 Jun 9;11:734. doi: 10.3389/fpls.2020.00734. eCollection 2020.
4
Alfalfa Root Growth Rate Correlates with Progression of Microtubules during Mitosis and Cytokinesis as Revealed by Environmental Light-Sheet Microscopy.环境光片显微镜揭示，苜蓿根生长速率与有丝分裂和胞质分裂过程中微管的进展相关。
Front Plant Sci. 2017 Oct 30;8:1870. doi: 10.3389/fpls.2017.01870. eCollection 2017.
5
Advanced Microscopy Reveals Complex Developmental and Subcellular Localization Patterns of ANNEXIN 1 in .先进显微镜技术揭示了膜联蛋白1在……中的复杂发育和亚细胞定位模式。
Front Plant Sci. 2020 Aug 5;11:1153. doi: 10.3389/fpls.2020.01153. eCollection 2020.
6
Light Sheet Fluorescence Microscopy Optimized for Long-Term Imaging of Arabidopsis Root Development.针对拟南芥根系发育长期成像优化的光片荧光显微镜技术。
Methods Mol Biol. 2018;1761:145-163. doi: 10.1007/978-1-4939-7747-5_11.
7
Advanced microscopy methods for bioimaging of mitotic microtubules in plants.用于植物有丝分裂微管生物成像的先进显微镜方法。
Methods Cell Biol. 2018;145:129-158. doi: 10.1016/bs.mcb.2018.03.019. Epub 2018 Apr 26.
8
Quantitation of cellular dynamics in growing Arabidopsis roots with light sheet microscopy.利用光片显微镜定量分析生长中的拟南芥根细胞动力学。
PLoS One. 2011;6(6):e21303. doi: 10.1371/journal.pone.0021303. Epub 2011 Jun 22.
9
Developmental Nuclear Localization and Quantification of GFP-Tagged EB1c in Arabidopsis Root Using Light-Sheet Microscopy.利用光片显微镜对拟南芥根中绿色荧光蛋白标记的EB1c进行发育核定位与定量分析
Front Plant Sci. 2016 Jan 5;6:1187. doi: 10.3389/fpls.2015.01187. eCollection 2015.
10
Light Sheet Fluorescence Microscopy Quantifies Calcium Oscillations in Root Hairs of Arabidopsis thaliana.光片荧光显微镜定量分析拟南芥根毛中的钙振荡
Plant Cell Physiol. 2017 Jul 1;58(7):1161-1172. doi: 10.1093/pcp/pcx045.

本文引用的文献

1
Mechanical control of cell proliferation patterns in growing epithelial monolayers.机械控制生长上皮单层细胞增殖模式。
Biophys J. 2024 Apr 2;123(7):909-919. doi: 10.1016/j.bpj.2024.03.002. Epub 2024 Mar 6.
2
Recent advances in understanding of the epigenetic regulation of plant regeneration.植物再生表观遗传调控研究的最新进展
aBIOTECH. 2023 Jan 16;4(1):31-46. doi: 10.1007/s42994-022-00093-2. eCollection 2023 Mar.
3
How do plants reprogramme the fate of differentiated cells?植物如何重新编程分化细胞的命运？
Curr Opin Plant Biol. 2023 Aug;74:102377. doi: 10.1016/j.pbi.2023.102377. Epub 2023 May 9.
4
A random-sampling approach to track cell divisions in time-lapse fluorescence microscopy.一种用于在延时荧光显微镜下追踪细胞分裂的随机抽样方法。
Plant Methods. 2021 Mar 8;17(1):25. doi: 10.1186/s13007-021-00723-8.
5
Novel Imaging Modalities Shedding Light on Plant Biology: Start Small and Grow Big.新型成像技术为植物生物学带来新曙光：从小处着手，逐步发展壮大。
Annu Rev Plant Biol. 2020 Apr 29;71:789-816. doi: 10.1146/annurev-arplant-050718-100038. Epub 2020 Mar 2.
6
Automated Imaging, Tracking, and Analytics Pipeline for Differentiating Environmental Effects on Root Meristematic Cell Division.用于区分环境对根分生组织细胞分裂影响的自动化成像、跟踪和分析流程
Front Plant Sci. 2019 Nov 19;10:1487. doi: 10.3389/fpls.2019.01487. eCollection 2019.
7
Week-long imaging of cell divisions in the Arabidopsis root meristem.对拟南芥根分生组织中细胞分裂进行为期一周的成像。
Plant Methods. 2019 Mar 25;15:30. doi: 10.1186/s13007-019-0417-9. eCollection 2019.
8
To regenerate or not to regenerate: factors that drive plant regeneration.是否再生：驱动植物再生的因素。
Curr Opin Plant Biol. 2019 Feb;47:138-150. doi: 10.1016/j.pbi.2018.12.002. Epub 2019 Jan 29.
9
Light Sheet Fluorescence Microscopy Optimized for Long-Term Imaging of Arabidopsis Root Development.针对拟南芥根系发育长期成像优化的光片荧光显微镜技术。
Methods Mol Biol. 2018;1761:145-163. doi: 10.1007/978-1-4939-7747-5_11.
10
3D analysis of mitosis distribution highlights the longitudinal zonation and diarch symmetry in proliferation activity of the Arabidopsis thaliana root meristem.三维分析有丝分裂分布突出了拟南芥根分生组织增殖活性的纵向分区和二项对称性。
Plant J. 2017 Dec;92(5):834-845. doi: 10.1111/tpj.13720. Epub 2017 Oct 20.