Baesso Paolo, Randall Ricardo S, Sena Giovanni
Department of Life Sciences, Imperial College London, London, UK.
Methods Mol Biol. 2018;1761:145-163. doi: 10.1007/978-1-4939-7747-5_11.
Light sheet fluorescence microscopy (LSFM) allows sustained and repeated optical sectioning of living specimens at high spatial and temporal resolution, with minimal photodamage. Here, we describe in detail both the hardware and the software elements of a live imaging method based on LSFM and optimized for tracking and 3D scanning of Arabidopsis root tips grown vertically in physiological conditions. The system is relatively inexpensive and with minimal footprint; hence it is well suited for laboratories of any size.
光片荧光显微镜（LSFM）能够以高空间和时间分辨率对活体标本进行持续且重复的光学切片，同时光损伤最小。在此，我们详细描述了一种基于LSFM的活体成像方法的硬件和软件要素，该方法针对在生理条件下垂直生长的拟南芥根尖的追踪和三维扫描进行了优化。该系统成本相对较低且占地面积最小；因此，它非常适合任何规模的实验室。
