Wilson D B
Arch Otorhinolaryngol. 1985;241(2):203-8. doi: 10.1007/BF00454355.
Homozygous loop-tail (Lp/Lp) mice exhibit defects in the otocyst as well as extensive neural dysraphism. At 19 days of gestation, cells in the otic pit of abnormal embryos are flattened and lack the rounded luminal contours characteristic of otic cells in their normal littermates. Apical filaments also are not as prominent as in normal embryos, and there is an increase in densely stained globular material in cells at the ventral lip of the otic pit. With glutaraldehyde-tannic acid fixation, the basal lamina of the otic pit cells shows differences from that of the normal otic pit. In abnormal specimens, the lamina densa is irregular and clumped, and the adjacent less dense area is spotty and lacks the more uniformly arranged and delicate fibrils characteristic of the normal basal lamina. These defects may reflect faulty developmental interactions between the dysraphic neural tube, mesenchymal cells, and otic anlage.
纯合环尾（Lp/Lp）小鼠在耳囊中表现出缺陷，同时伴有广泛的神经管闭合不全。在妊娠19天时，异常胚胎的耳窝中的细胞扁平，缺乏正常同窝仔鼠耳细胞特有的圆形管腔轮廓。顶端细丝也不如正常胚胎中明显，并且在耳窝腹侧唇的细胞中，密集染色的球状物质有所增加。用戊二醛 - 单宁酸固定后，耳窝细胞的基膜与正常耳窝的基膜存在差异。在异常标本中，致密层不规则且聚集，相邻的较疏松区域有斑点，缺乏正常基膜特有的排列更均匀且精细的纤维。这些缺陷可能反映了神经管闭合不全、间充质细胞和耳原基之间发育相互作用的异常。
