Chlibek Roman, Smetana Jan, Kybicová Kateřina, Malikova Martina, Angulo Frederick J, Loew-Baselli Alexandra, Tan Ye, Ondřejíček Aleš, Brestrich Gordon, Pilz Andreas, Moïsi Jennifer C, Stark James H
Military Medical Faculty, University of Defence, Trebesska 1575, Hradec Kralove 500 01, Czech Republic.
National Reference Laboratory for Lyme Borreliosis, National Health Institute, Šrobárova 49/48, Prague 10, 100 00, Czech Republic.
Int J Med Microbiol. 2025 Mar;318:151644. doi: 10.1016/j.ijmm.2024.151644. Epub 2025 Jan 3.
Lyme borreliosis (LB), an infection caused by Borrelia burgdorferi sensu lato (Bbsl), is the most common tick-borne disease in Europe. To further characterize the LB burden in the Czech Republic, we conducted a seroprevalence study and estimated the incidence of symptomatic Bbsl infections.
Anti-Bbsl IgM and IgG antibodies were detected in sera collected from the adult population in 2011 -2012 by enzyme-linked immunosorbent assay and immunoblot tests at the National Reference Laboratory. The incidence of symptomatic Bbsl infections was estimated from the seroprevalence results and the symptomatic proportion and duration of persistence of anti-Bbsl IgG antibodies in Bbsl-infected individuals. Surveillance under-detection of symptomatic Bbsl infections was estimated by comparing surveillance-reported and seroprevalence-based incidence.
Samples from 1996 adults were tested; the median age (range) was 45 (18 -87) years; 1037 (52.0 %) were female. The prevalence (with 95 % confidence interval) of anti-Bbsl IgG, and IgM and/or IgG (IgM/IgG) antibodies was 6.3 % (5.3 -7.5 %), and 9.5 % (8.3 -10.9 %), respectively. The IgM/IgG prevalence was 7.8 % (6.5 -9.2 %) in Bohemia and 15.3 % (12.2 -19.0 %) in Moravia. There were an estimated 30,563 (26,550 -34,962) symptomatic incident Bbsl infections in adults in the Czech Republic in 2012, for an incidence of 352.2 (306.0 -402.9) symptomatic Bbsl infections per 100,000 adults per year. There were an estimated 11 (10 -13) symptomatic Bbsl infections for each surveillance-reported LB case in the Czech Republic in 2012.
There is high incidence of symptomatic Bbsl infections in the Czech Republic, particularly in Moravia. Interventions are needed to address the substantial burden of LB in the Czech Republic.
莱姆病（LB）是一种由伯氏疏螺旋体复合群（Bbsl）引起的感染，是欧洲最常见的蜱传疾病。为了进一步描述捷克共和国的莱姆病负担，我们开展了一项血清流行率研究，并估计了有症状的Bbsl感染的发病率。
2011年至2012年期间，在国家参考实验室通过酶联免疫吸附测定和免疫印迹试验，检测从成年人群中采集的血清中的抗Bbsl IgM和IgG抗体。根据血清流行率结果以及Bbsl感染个体中抗Bbsl IgG抗体的有症状比例和持续时间，估计有症状的Bbsl感染的发病率。通过比较监测报告的发病率和基于血清流行率的发病率，估计有症状的Bbsl感染的监测漏报情况。
对1996名成年人的样本进行了检测；年龄中位数（范围）为45（18 - 87）岁；1037名（52.0%）为女性。抗Bbsl IgG、IgM和/或IgG（IgM/IgG）抗体的流行率（95%置信区间）分别为6.3%（5.3 - 7.5%）和9.5%（8.3 - 10.9%）。IgM/IgG流行率在波希米亚为7.8%（6.5 - 9.2%），在摩拉维亚为15.3%（12.2 - 19.0%）。2012年，捷克共和国成年人中有症状的Bbsl感染发病例数估计为30563例（26550 - 34962例），发病率为每10万成年人每年352.2例（306.0 - 402.9例）有症状的Bbsl感染。2012年，在捷克共和国，每例监测报告的莱姆病病例估计对应11例（10 - 13例）有症状的Bbsl感染。
捷克共和国有症状的Bbsl感染发病率很高，尤其是在摩拉维亚。需要采取干预措施来应对捷克共和国莱姆病的沉重负担。
