Kybicová K, Kurzová Z, Hulínská D
National Reference Laboratory for Lyme Borreliosis, National Institute of Public Health, Prague, Czech Republic.
Vector Borne Zoonotic Dis. 2008 Oct;8(5):645-52. doi: 10.1089/vbz.2007.0249.
The aim of the present study was to determine the frequency and spatial distribution of the Borrelia species in wild rodents in the Czech Republic. In total, 293 muscle tissue samples and 106 sera from 293 wild rodents captured in North Bohemia and North-East and South Moravia were examined for the presence of Borrelia spp. and antibodies. Muscle samples were investigated with real-time polymerase chain reaction (PCR) with a recA primer set, with DNA quantification and melting curve analysis, and with restriction fragment length polymorphism (RFLP) analysis of the 5S-23S rDNA intergenic spacer. Infection with Borrelia burgdorferi sensu lato was found in 16.4% of the muscle samples. The most abundant genospecies was Borrelia afzelii (11.3%), followed by Borrelia burgdorferi sensu stricto (4.8%) and Borrelia garinii (0.7%). Borrelia infection was more frequently observed in Clethrionomys glareolus than in Apodemus spp. Sera were analyzed with an enzyme-linked immunosorbent assay (ELISA) test, yielding the total seropositivity rates of 24.5% for anti-Borrelia IgM antibodies and 25.5% for IgG antibodies. Total seroprevalence was higher in Apodemus spp. than in C. glareolus. In conclusion, our data indicate that in the Czech Republic small wild rodents can serve as hosts for B. burgdorferi s. s. as well as for B. afzelii.
本研究的目的是确定捷克共和国野生啮齿动物中疏螺旋体属物种的频率和空间分布。总共对从北波希米亚、摩拉维亚东北部和南部捕获的293只野生啮齿动物的293份肌肉组织样本和106份血清进行了检测，以查找疏螺旋体属细菌和抗体的存在情况。肌肉样本采用recA引物组通过实时聚合酶链反应（PCR）进行检测，并进行DNA定量和熔解曲线分析，以及对5S - 23S rDNA基因间隔区进行限制性片段长度多态性（RFLP）分析。在16.4%的肌肉样本中发现感染了广义伯氏疏螺旋体。最常见的基因种是阿氏疏螺旋体（11.3%），其次是狭义伯氏疏螺旋体（4.8%）和伽氏疏螺旋体（0.7%）。在棕背䶄中观察到的疏螺旋体感染比姬鼠属更频繁。血清通过酶联免疫吸附测定（ELISA）试验进行分析，抗疏螺旋体IgM抗体的总血清阳性率为24.5%，IgG抗体为25.5%。姬鼠属的总血清阳性率高于棕背䶄。总之，我们的数据表明，在捷克共和国，小型野生啮齿动物可以作为狭义伯氏疏螺旋体以及阿氏疏螺旋体的宿主。
