Sedaghat Asghar, Karimi Torshizi Mohammad Amir, Soleimani Mohammad Reza
Department of Poultry Science, University of Georgia, Athens, GA 30602, USA; Department of Poultry Science, College of Agriculture, Tarbiat Modares University, Tehran, Iran 14115336.
Department of Poultry Science, College of Agriculture, Tarbiat Modares University, Tehran, Iran 14115336.
Poult Sci. 2025 Feb;104(2):104741. doi: 10.1016/j.psj.2024.104741. Epub 2024 Dec 26.
This study was conducted to evaluate the effects of E.coli Nissle 1917 (EcN) on immune responses, blood parameters, oxidative stress, egg quality, and performance of laying Japanese quail. A total of one-hundred day-old quail chicks were assigned to 1 of 4 treatments based on probiotic concentration: 1 (0 CFU/mL; control), 2 (10 CFU/mL), 3 (10 CFU/mL), and 4 (10 CFU/mL). The average egg production throughout the 8 wks of the laying phase increased with the increase in EcN supplementation (R= 0.96). The egg production was also significantly different in wk 4, 7, and 8 of the laying phase with the control group demonstrating the poorest performance. External egg quality parameters, including shell strength, Roche index, albumen height, yolk weight, shell weight, shell thickness, and Haugh unit, also showed higher values in groups receiving EcN. Likewise, these groups demonstrated heavier egg weight (EW) and lower MDA in the meat sample. The MDA concentration of fresh yolk samples in groups receiving EcN was higher but diminished after iron-inducing (P < 0.05). No differences were observed in ADFI, FCR, EM, yolk cholesterol and triglyceride, hematocrit, SRBC, NDV, CMI immune responses (DNCB and PHA), H:L ratio, and internal organ weights. Plasma cholesterol and uric acid were the only blood constituents showing higher values in male birds in groups of EcN (P < 0.05); no appreciable differences were observed in the rest of the blood parameters in male birds and none in females. Further, a substantially higher Avian Influenza Virus (AIV) titer resulted from treatments 3 and 4 in male birds among humoral immune responses. These results demonstrated that EcN in the birds' drinking water could profoundly influence laying performance, egg quality, immune function, and enhanced antioxidant capacity.
本研究旨在评估大肠杆菌Nissle 1917（EcN）对日本蛋鹌鹑免疫反应、血液参数、氧化应激、蛋品质及生产性能的影响。根据益生菌浓度，将总共100只1日龄鹌鹑雏鸡分为4种处理组之一：1组（0 CFU/mL；对照组）、2组（10⁶ CFU/mL）、3组（10⁷ CFU/mL）和4组（10⁸ CFU/mL）。产蛋期8周内的平均产蛋量随EcN添加量的增加而增加（R = 0.96）。产蛋期第4、7和8周的产蛋量也存在显著差异，对照组表现最差。包括蛋壳强度、罗氏指数、蛋白高度、蛋黄重量、蛋壳重量、蛋壳厚度和哈夫单位在内的外部蛋品质参数，在接受EcN的组中也呈现出更高的值。同样，这些组的蛋重（EW）更重，肉样中的丙二醛（MDA）含量更低。接受EcN的组中新鲜蛋黄样本的MDA浓度较高，但在铁诱导后降低（P < 0.05）。在平均日采食量（ADFI）、料蛋比（FCR）、蛋重（EM）、蛋黄胆固醇和甘油三酯、红细胞压积、SRBC、新城疫病毒（NDV）、细胞介导免疫反应（二硝基氯苯和PHA）、H:L比值以及内脏器官重量方面未观察到差异。血浆胆固醇和尿酸是EcN组雄性鸟类中唯一显示较高值的血液成分（P < 0.05）；雄性鸟类其余血液参数未观察到明显差异，雌性鸟类也无差异。此外，在体液免疫反应中，处理3组和4组的雄性鸟类产生的禽流感病毒（AIV）滴度显著更高。这些结果表明，鸟类饮用水中的EcN可深刻影响产蛋性能、蛋品质、免疫功能并增强抗氧化能力。
