Madison Imani, Moreno-Risueno Miguel, Sozzani Rosangela
Department of Plant and Microbial Biology and NC Plant Sciences Initiative, North Carolina State University, Raleigh, NC 27695, USA.
Centro de Biotecnología y Genómica de Plantas (Universidad Politécnica de Madrid [UPM] - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, CSIC [INIA-CSIC]), Madrid, Spain.
Curr Opin Biotechnol. 2025 Feb;91:103250. doi: 10.1016/j.copbio.2024.103250. Epub 2025 Jan 8.
The integration of 3D bioprinting into plant science and biotechnology is revolutionizing research and applications. While many high-throughput techniques have advanced plant biology, replicating the complex 3D organization and cellular environments of plant tissues remains a significant challenge. Traditional 2D culture systems fall short of capturing the necessary spatial context for accurate studies of cell behavior, gene expression, and tissue development. Additionally, the lack of precise simulation of plant microenvironments limits control over cellular interactions and responses to external stimuli. Recent advancements in 3D bioprinting address these limitations by allowing precise control over cell positioning and biomaterial arrangement, thereby better replicating natural plant environments. This enables more accurate studies of gene expression, developmental processes, and stress responses. The technology also enhances our ability to test genetic modifications and biotechnological interventions, advancing crop improvement, sustainable agriculture, and precision breeding. This review examines the current state of 3D bioprinting in plant science, discusses its limitations, and explores its potential to transform research and applications in the field.
将3D生物打印技术整合到植物科学与生物技术领域正在彻底改变相关研究与应用。尽管许多高通量技术推动了植物生物学的发展，但复制植物组织复杂的3D结构和细胞环境仍然是一项重大挑战。传统的二维培养系统无法为准确研究细胞行为、基因表达和组织发育提供必要的空间背景。此外，对植物微环境缺乏精确模拟限制了对细胞间相互作用以及对外界刺激反应的控制。3D生物打印技术的最新进展通过实现对细胞定位和生物材料排列的精确控制来克服这些限制，从而更好地复制天然植物环境。这使得对基因表达、发育过程和应激反应的研究更加准确。该技术还增强了我们测试基因改造和生物技术干预措施的能力，推动了作物改良、可持续农业和精准育种的发展。本文综述了植物科学中3D生物打印技术的现状，讨论了其局限性，并探讨了该技术在改变该领域研究与应用方面的潜力。
