饥饿以及用高碳水化合物饮食喂养会引起大鼠肝脏丙酮酸激酶比活性的变化。
Starvation and feeding with a high-carbohydrate diet induce changes in the specific activity of rat hepatic pyruvate kinase.
作者信息
Cimbala M A, Lau D, Daigneault J F
出版信息
Biochem J. 1985 Feb 15;226(1):299-303. doi: 10.1042/bj2260299.
Abstract
A highly specific radiosandwich assay for hepatic type-L pyruvate kinase (PK) determinants was developed. This assay was used to evaluate changes in PK specific activity which occur on starvation or feeding with a high-carbohydrate diet. Despite a large increase in both catalytic activity and immunoreactive protein, the calculated specific activity falls on feeding, and the opposite effect occurs on starvation. These change can be attributed to disproportionate changes in immunoreactive protein compared with changes in catalytic activity.
摘要
开发了一种用于肝型L丙酮酸激酶（PK）决定簇的高特异性放射夹心测定法。该测定法用于评估饥饿或高碳水化合物饮食喂养时PK比活性的变化。尽管催化活性和免疫反应性蛋白均大幅增加，但计算得出的比活性在进食时下降，而在饥饿时则出现相反的效果。这些变化可归因于免疫反应性蛋白的变化与催化活性的变化不成比例。
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Biochim Biophys Acta. 1982 Mar 29;696(3):340-4. doi: 10.1016/0167-4781(82)90066-5.
3
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4
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