一种在体外将放射性标记胆固醇酯掺入人血浆脂蛋白的替代方法。
An alternative procedure for incorporating radiolabelled cholesteryl ester into human plasma lipoproteins in vitro.
作者信息
Roberts D C, Miller N E, Price S G, Crook D, Cortese C, La Ville A, Masana L, Lewis B
出版信息
Biochem J. 1985 Feb 15;226(1):319-22. doi: 10.1042/bj2260319.
Abstract
A simple method has been developed for labelling human plasma lipoproteins to high specific radioactivity with radioactive cholesteryl esters in vitro. After isolation by preparative ultracentrifugation, the selected lipoprotein was incubated for 30 min at 4 degrees C in human serum (d greater than 1.215) that had been prelabelled with [4-14C]cholesteryl oleate or [1,2-3H]cholesteryl linoleate, and was then re-isolated by ultracentrifugation. All major lipoprotein classes were labelled by the procedure. Specific radioactivities of up to 18 d.p.m. . pmol-1 (46 d.p.m. . ng-1) were achieved. When radiolabelled high-density lipoprotein was infused intravenously, the radioactive cholesteryl ester behaved in vivo indistinguishably from endogenous cholesteryl esters produced by the lecithin (phosphatidylcholine): cholesterol acyltransferase reaction.
摘要
已开发出一种简单方法，可在体外将人血浆脂蛋白用放射性胆固醇酯标记至高比放射性。通过制备性超速离心分离后，将选定的脂蛋白在4℃下于已用[4-¹⁴C]胆固醇油酸酯或[1,2-³H]胆固醇亚油酸酯预标记的人血清（d大于1.215）中孵育30分钟，然后通过超速离心重新分离。所有主要脂蛋白类别均通过该程序进行标记。比放射性可达18 d.p.m.·pmol⁻¹（46 d.p.m.·ng⁻¹）。当静脉内注入放射性标记的高密度脂蛋白时，放射性胆固醇酯在体内的行为与卵磷脂（磷脂酰胆碱）：胆固醇酰基转移酶反应产生的内源性胆固醇酯无明显区别。
相似文献
1
An alternative procedure for incorporating radiolabelled cholesteryl ester into human plasma lipoproteins in vitro.一种在体外将放射性标记胆固醇酯掺入人血浆脂蛋白的替代方法。
Biochem J. 1985 Feb 15;226(1):319-22. doi: 10.1042/bj2260319.
2
Preparation of high-density lipoproteins labelled exclusively at the cholesteryl ester moiety.仅在胆固醇酯部分标记的高密度脂蛋白的制备。
Clin Chim Acta. 1982 Aug 4;123(1-2):139-44. doi: 10.1016/0009-8981(82)90122-x.
3
Comparison of various methods for in vitro cholesteryl ester labeling of lipoproteins from hypercholesterolemic rabbits.高胆固醇血症兔脂蛋白体外胆固醇酯标记的各种方法比较
Biochim Biophys Acta. 1984 Mar 7;792(3):338-47. doi: 10.1016/0005-2760(84)90202-9.
4
[3H]Cholesteryl ester labeling and transfer among human and nonhuman primate plasma lipoproteins.[3H]胆固醇酯在人类和非人类灵长类动物血浆脂蛋白中的标记与转移
Anal Biochem. 1983 Apr 1;130(1):215-22. doi: 10.1016/0003-2697(83)90672-3.
5
6
A method for direct incorporation of radiolabeled cholesteryl ester into human plasma low-density-lipoproteins: preparation of tracer substrate for cholesteryl ester transfer reaction between lipoproteins.一种将放射性标记的胆固醇酯直接掺入人血浆低密度脂蛋白的方法：用于脂蛋白间胆固醇酯转移反应的示踪底物的制备。
J Biochem. 1986 Jan;99(1):295-301. doi: 10.1093/oxfordjournals.jbchem.a135473.
7
High-density lipoprotein subpopulations as substrates for the transfer of cholesteryl esters to very-low-density lipoproteins.高密度脂蛋白亚群作为胆固醇酯向极低密度脂蛋白转移的底物。
Biochem J. 1990 Sep 1;270(2):441-9. doi: 10.1042/bj2700441.
8
Comparison of the metabolism of [1,2,6,7-3H(N)]cholesteryl oleate, cholesteryl [9,10-3H]oleate, and cholesteryl [1-14C]oleate labeled lipoproteins in the rat.
Atherosclerosis. 1994 Apr;106(2):203-11. doi: 10.1016/0021-9150(94)90125-2.
9
In vivo transfer of cholesteryl ester from high and low density plasma lipoproteins into human aortic tissue.胆固醇酯在体内从高密度和低密度血浆脂蛋白向人主动脉组织的转移。
Arteriosclerosis. 1988 May-Jun;8(3):252-62. doi: 10.1161/01.atv.8.3.252.
10
In vitro incorporation of cholesterol-14C into very low density lipoprotein cholesteryl esters.胆固醇-14C在体外掺入极低密度脂蛋白胆固醇酯中。
J Lipid Res. 1968 Sep;9(5):620-6.
引用本文的文献
1
Hepatocyte ATF3 protects against atherosclerosis by regulating HDL and bile acid metabolism.肝细胞激活转录因子 3 通过调节高密度脂蛋白和胆汁酸代谢来保护动脉粥样硬化。
Nat Metab. 2021 Jan;3(1):59-74. doi: 10.1038/s42255-020-00331-1. Epub 2021 Jan 18.
2
Simultaneous Determination of Biliary and Intestinal Cholesterol Secretion Reveals That CETP (Cholesteryl Ester Transfer Protein) Alters Elimination Route in Mice.同时测定胆汁和肠道胆固醇分泌揭示 CETP（胆固醇酯转移蛋白）改变了小鼠的消除途径。
Arterioscler Thromb Vasc Biol. 2019 Oct;39(10):1986-1995. doi: 10.1161/ATVBAHA.119.312952. Epub 2019 Aug 29.
3
Global inactivation of carboxylesterase 1 (Ces1/Ces1g) protects against atherosclerosis in Ldlr mice.羧酯酶 1（Ces1/Ces1g）的全球失活可保护 LDLR 小鼠免受动脉粥样硬化的影响。
Sci Rep. 2017 Dec 19;7(1):17845. doi: 10.1038/s41598-017-18232-x.
4
Streptococcal serum opacity factor promotes cholesterol ester metabolism and bile acid secretion in vitro and in vivo.链球菌血清混浊因子在体外和体内均能促进胆固醇酯代谢及胆汁酸分泌。
Biochim Biophys Acta. 2016 Mar;1861(3):196-204. doi: 10.1016/j.bbalip.2015.12.006. Epub 2015 Dec 18.
5
Scavenger receptor class B, type I (Scarb1) deficiency promotes osteoblastogenesis but stunts terminal osteocyte differentiation.I型清道夫受体B类（Scarb1）缺乏促进成骨细胞生成，但阻碍终末骨细胞分化。
Physiol Rep. 2014 Oct 2;2(10). doi: 10.14814/phy2.12117. Print 2014 Oct 1.
6
The atherogenic Scarb1 null mouse model shows a high bone mass phenotype.载脂蛋白 B 编辑酶 1 缺失小鼠模型表现出高骨量表型。
Am J Physiol Endocrinol Metab. 2014 Jan 1;306(1):E48-57. doi: 10.1152/ajpendo.00421.2013. Epub 2013 Nov 19.
7
Identification of a PDZ-domain-containing protein that interacts with the scavenger receptor class B type I.一种与I型B类清道夫受体相互作用的含PDZ结构域蛋白的鉴定。
Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6538-43. doi: 10.1073/pnas.100114397.
8
Effect of reduced low-density lipoprotein receptor level on HepG2 cell cholesterol metabolism.低密度脂蛋白受体水平降低对HepG2细胞胆固醇代谢的影响。
Biochem J. 1998 Jan 1;329 ( Pt 1)(Pt 1):81-9. doi: 10.1042/bj3290081.
9
Selective uptake of cholesteryl esters of low-density lipoproteins is mediated by the lipoprotein-binding site in HepG2 cells and is followed by the hydrolysis of cholesteryl esters.低密度脂蛋白胆固醇酯的选择性摄取由HepG2细胞中的脂蛋白结合位点介导，随后是胆固醇酯的水解。
Biochem J. 1996 Sep 15;318 ( Pt 3)(Pt 3):841-7. doi: 10.1042/bj3180841.
10
Prevention by alpha-tocopherol and rutin of glutathione and ATP depletion induced by oxidized LDL in cultured endothelial cells.α-生育酚和芦丁对培养的内皮细胞中氧化型低密度脂蛋白诱导的谷胱甘肽和三磷酸腺苷耗竭的预防作用
Br J Pharmacol. 1995 Oct;116(3):1985-90. doi: 10.1111/j.1476-5381.1995.tb16402.x.
本文引用的文献
1
A simple method for the isolation and purification of total lipides from animal tissues.一种从动物组织中分离和纯化总脂质的简单方法。
J Biol Chem. 1957 May;226(1):497-509.
2
Kinetic studies of the transfer of esterified cholesterol between human plasma low and high density lipoproteins.人血浆低密度和高密度脂蛋白间酯化胆固醇转移的动力学研究
J Lipid Res. 1980 Feb;21(2):238-49.
3
Comparison of different methods of isotopically labelling the esterified cholesterol in human high density lipoproteins.人体高密度脂蛋白中酯化胆固醇不同同位素标记方法的比较。
Biochim Biophys Acta. 1982 Mar 12;710(3):349-58. doi: 10.1016/0005-2760(82)90118-7.
4
[3H]Cholesteryl ester labeling and transfer among human and nonhuman primate plasma lipoproteins.[3H]胆固醇酯在人类和非人类灵长类动物血浆脂蛋白中的标记与转移
Anal Biochem. 1983 Apr 1;130(1):215-22. doi: 10.1016/0003-2697(83)90672-3.
5
Comparison of various methods for in vitro cholesteryl ester labeling of lipoproteins from hypercholesterolemic rabbits.高胆固醇血症兔脂蛋白体外胆固醇酯标记的各种方法比较
Biochim Biophys Acta. 1984 Mar 7;792(3):338-47. doi: 10.1016/0005-2760(84)90202-9.
6
Individual variation in the effects of dietary cholesterol on plasma lipoproteins and cellular cholesterol homeostasis in man. Studies of low density lipoprotein receptor activity and 3-hydroxy-3-methylglutaryl coenzyme A reductase activity in blood mononuclear cells.饮食胆固醇对人体血浆脂蛋白和细胞胆固醇稳态影响的个体差异。血液单核细胞中低密度脂蛋白受体活性和3-羟基-3-甲基戊二酰辅酶A还原酶活性的研究。
J Clin Invest. 1981 Feb;67(2):493-502. doi: 10.1172/JCI110058.
7
Determination of lecithin: cholesterol acyltransfer in human blood plasma.人血浆中卵磷脂：胆固醇酰基转移酶的测定
Scand J Clin Lab Invest. 1971 Feb;27(1):21-7. doi: 10.3109/00365517109080184.
8
9
Receptor-dependent hydrolysis of cholesteryl esters contained in plasma low density lipoprotein.血浆低密度脂蛋白中胆固醇酯的受体依赖性水解。
Proc Natl Acad Sci U S A. 1975 Aug;72(8):2925-9. doi: 10.1073/pnas.72.8.2925.
Zotero 插件