Samollow P B, VandeBerg J L, Kunz H W, Gill T J
Biochem Biophys Res Commun. 1985 Feb 15;126(3):1182-8. doi: 10.1016/0006-291x(85)90310-9.
A simple cellulose acetate electrophoretic method for visualizing mammalian neuraminidase isozymes has been developed. Application of the method with rat and mouse liver extracts reveals the presence of two distinct isozymes in each species. Each isozyme exhibits tremendous variation in activity between inbred strains. The two isozymes vary independently of one another suggesting that their activities are controlled by different genes. The neuraminidase phenotypes detected in these inbred strains via electrophoresis are consistent with published accounts of neuraminidase phenotypes determined fluorometrically in whole liver homogenates, but also indicate the presence of a second isozyme not perceived by this other procedure.
已开发出一种用于可视化哺乳动物神经氨酸酶同工酶的简单醋酸纤维素电泳方法。将该方法应用于大鼠和小鼠肝脏提取物，结果显示每个物种中存在两种不同的同工酶。每种同工酶在近交系之间的活性表现出巨大差异。这两种同工酶彼此独立变化，表明它们的活性由不同基因控制。通过电泳在这些近交系中检测到的神经氨酸酶表型与在全肝匀浆中通过荧光测定法确定的神经氨酸酶表型的已发表报道一致，但也表明存在另一种方法未检测到的第二种同工酶。
