一种新的脑髓鞘蛋白：分离与化学特性分析。

A new protein of the brain myelin: isolation and chemical characterization.

作者信息

Riccio P, Tsugita A, Bobba A, Vilbois F, Quagliariello E

出版信息

Biochem Biophys Res Commun. 1985 Mar 15;127(2):484-92. doi: 10.1016/s0006-291x(85)80186-8.

Abstract

An unknown protein has been isolated from bovine brain myelin. This protein, purified in the nonionic detergent n-octylpolydisperse oligooxyethylene, reveals on SDS gel electrophoresis a large number of bands in the higher MW region. However, chemical analysis and gel chromatography indicate the presence of a single, small protein containing large amounts of bound phosphatidylserine. N-terminal and C-terminal sequences, aminoacid composition, and the anomalous electrophoretic behaviour led us to exclude the protein as a fragment of other already known myelin proteins.

摘要

一种未知蛋白质已从牛脑髓磷脂中分离出来。这种在非离子洗涤剂正辛基多分散聚氧乙烯中纯化的蛋白质，在SDS凝胶电泳上显示出较高分子量区域有大量条带。然而，化学分析和凝胶色谱表明存在一种单一的小蛋白质，其含有大量结合的磷脂酰丝氨酸。N端和C端序列、氨基酸组成以及异常的电泳行为使我们排除该蛋白质是其他已知髓磷脂蛋白质的片段。