Pottenger L A, Webb J E, Lyon N B
Arthritis Rheum. 1985 Mar;28(3):323-30. doi: 10.1002/art.1780280313.
Fifty-one uniformly sliced human patellar cartilage specimens were extracted with physiologic buffer containing inhibitors. Galactosamine-rich proteoglycans were more easily extracted than glucosamine-rich proteoglycans. Extraction occurred in 2 phases. There was a large proteoglycan extraction during the first 30 minutes, followed by a steady loss proportional to the square root of time. The amount of proteoglycans extracted during phase 1 was approximately half that of phase 2. Extraction of proteoglycans varied with age, structural integrity, and cartilage thickness. Intact osteoarthritic cartilage had normal proteoglycan extraction.
用含有抑制剂的生理缓冲液提取了51个均匀切片的人髌软骨标本。富含半乳糖胺的蛋白聚糖比富含葡萄糖胺的蛋白聚糖更容易被提取。提取分两个阶段进行。在最初的30分钟内有大量的蛋白聚糖被提取，随后以与时间平方根成正比的稳定速率流失。第一阶段提取的蛋白聚糖量约为第二阶段的一半。蛋白聚糖的提取随年龄、结构完整性和软骨厚度而变化。完整的骨关节炎软骨具有正常的蛋白聚糖提取。
