Liu Sainan, Wei Lai, Huang Jinquan, Luo Jiayan, Weng Yajun, Chen Junying
Key Laboratory of Advanced Technology for Materials of Chinese Education Ministry, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, China.
Institute of Biomedical Engineering, College of Medicine, Southwest Jiaotong University, Chengdu, China.
J Biomed Mater Res B Appl Biomater. 2025 Jan;113(1):e35533. doi: 10.1002/jbm.b.35533.
Burns are complex traumatic injuries that lead to severe physical and psychological problems due to the prolonged healing period and resulting physical scars. Owing to their versatility, hydrogels can be loaded with various functional factors, making them promising wound dressings. However, many hydrogel dressings cannot support cell survival for a long time, thereby delaying the process of tissue repair. Herein, based on chitosan (CS)/alginate (SA)/poly(ethylene glycol) diacrylate (PEGDA), a basic hydrogel with hemostasis and antibacterial properties was prepared, and loaded with vascular endothelial cadherin (VE-cadherin) and fibroblast growth factors (FGF) to promote the co-culture of various skin cells, suitable for treating various skin injury types: (1) Construct a three-dimensional microenvironment conducive to the release of drugs and factors using natural biological macromolecules CS/SA. (2) Promote the cell growth by loading growth factors. (3) Establish skin burn models of different degrees and observe the repair process. From the results, the 3D microenvironment provided by hydrogel could support the active growth of cells for 12 days. Furthermore, deep burns with full-thickness skin were substantially repaired within about 24 days. Collectively, CS/SA hydrogel containing VE-cadherin and FGF can promote tissue healing in wounds with necrotic tissue, making it an ideal candidate for burn treatment.
烧伤是复杂的创伤性损伤，由于愈合期延长和由此产生的身体疤痕，会导致严重的身体和心理问题。由于其多功能性，水凝胶可以负载各种功能因子，使其成为有前景的伤口敷料。然而，许多水凝胶敷料不能长时间支持细胞存活，从而延迟了组织修复过程。在此，基于壳聚糖（CS）/海藻酸盐（SA）/聚乙二醇二丙烯酸酯（PEGDA）制备了一种具有止血和抗菌性能的基础水凝胶，并负载血管内皮钙黏蛋白（VE-钙黏蛋白）和成纤维细胞生长因子（FGF）以促进各种皮肤细胞的共培养，适用于治疗各种类型的皮肤损伤：（1）使用天然生物大分子CS/SA构建有利于药物和因子释放的三维微环境。（2）通过负载生长因子促进细胞生长。（3）建立不同程度的皮肤烧伤模型并观察修复过程。结果表明，水凝胶提供的三维微环境可以支持细胞活跃生长12天。此外，全层皮肤深度烧伤在约24天内基本得到修复。总体而言，含有VE-钙黏蛋白和FGF的CS/SA水凝胶可以促进有坏死组织伤口的组织愈合，使其成为烧伤治疗的理想候选材料。
