Zhang Zhike
Ningxia Academy of Agriculture and Forestry Sciences, Institute of Plant Protection, Yinchuan, China.
Bull Entomol Res. 2025 Feb;115(1):74-83. doi: 10.1017/S0007485324000683.
Insect odorant-binding proteins (OBPs) are the key proteins in insect olfactory perception and play an important role in the perception and discrimination of insects. is a polyphagous pest and seriously harms the quality and yield of fruits, flowers and crops worldwide. Therefore, the discovery of OBPs has greatly improved the understanding of behavioural response that mediates the chemoreception of . To identify the OBP gene of and its sequence and expression, rapid amplification cDNA ends (RACE) and qRT-PCR reaction system were performed. The results showed that the sequence of FoccOBP9 gene was 846 bp and the reading frame was 558 bp, encoding 185 amino acid residues, a 3' non-coding region of 195 bp and a 5' non-coding region of 93 bp.The molecular weight of the protein was about 20.08 kDa, and the isoelectric point was 8.89. FoccOBP9 was similar to AtumGOBP and CnipOBP2 (30%), followed by BdorGOBP, DficGOBP, DsuzGOBP, AalbOBP38, CmarOBP6 and SexiOBP. Phylogenetic analysis of the FoccOBP9 demonstrated that the FoccOBP9 had a relatively close evolutionary relationship with SgreOBP1, AtumGOBP, HeleOBP3, CbowOBP17, CnipOBP2 and CpalOBP2. The prediction of secondary structure showed that FoccOBP9 protein contained 135 amino acid residues forming α-helix, 91 amino acid residues forming β-sheets and 24 amino acid residues forming β-turning. However, three-dimensional structure prediction showed that the FoccOBP9 protein skeleton was composed of six α-helices and the loops connecting these helices. Dynamic observation of the three-dimensional structure revealed that five α-helices (α1, α2, α4, α5, α6) were found in the structure. The expression profiles analysis revealed that are highly abundant in antenna significantly, followed by the head and belly, and almost no expression in the chest and foot. Therefore, the identification and analysis of OBP may be useful for monitoring and limiting the damage of . .
昆虫气味结合蛋白（OBPs）是昆虫嗅觉感知中的关键蛋白，在昆虫的感知和辨别中发挥着重要作用。[某种昆虫名称]是一种多食性害虫，严重危害全球水果、花卉和农作物的质量与产量。因此，OBPs的发现极大地增进了人们对介导[该昆虫名称]化学感受的行为反应的理解。为了鉴定[该昆虫名称]的OBP基因及其序列和表达情况，进行了快速扩增cDNA末端（RACE）和qRT-PCR反应体系实验。结果表明，FoccOBP9基因序列为846 bp，阅读框为558 bp，编码185个氨基酸残基，3'非编码区为195 bp，5'非编码区为93 bp。该蛋白质的分子量约为20.08 kDa，等电点为8.89。FoccOBP9与AtumGOBP和CnipOBP2相似性为30%，其次与BdorGOBP、DficGOBP、DsuzGOBP、AalbOBP38、CmarOBP6和SexiOBP相似。对FoccOBP9的系统发育分析表明，FoccOBP9与SgreOBP1、AtumGOBP、HeleOBP3、CbowOBP17、CnipOBP2和CpalOBP2具有相对较近的进化关系。二级结构预测显示，FoccOBP9蛋白包含135个形成α螺旋的氨基酸残基、91个形成β折叠的氨基酸残基和24个形成β转角的氨基酸残基。然而，三维结构预测表明，FoccOBP9蛋白骨架由六个α螺旋以及连接这些螺旋的环组成。对三维结构的动态观察发现，该结构中有五个α螺旋（α1、α2、α4、α5、α6）。表达谱分析显示，[该昆虫名称]在触角中含量极高，其次是头部和腹部，在胸部和足部几乎不表达。因此，OBP的鉴定和分析可能有助于监测和限制[该昆虫名称]的危害。
