Rikmenspoel R, Isles C A
Biophys J. 1985 Mar;47(3):395-410. doi: 10.1016/S0006-3495(85)83931-X.
High speed cinemicrographs were made of sea urchin sperm at temperatures varying from 22 to 6 degrees C. Apparatus, combining a television camera and a video digitizer, was constructed to scan individual flagellar images and to digitize the flagellar waveforms. With appropriate smoothing and averaging procedures, the rough data were condensed by a microcomputer into the coordinates of 20 points along a flagellum, spaced 2 microns apart. The curvature of the flagellum at these points was also computed. The coordinates of the flagellar positions were obtained to an accuracy of approximately +/- 0.1 micron, flagellar curvature to an accuracy of approximately +/- 50 cm-1. At all temperatures the amplitude of the flagella was found to vary with time in a purely sinusoidal fashion to within +/- 2%. The local curvature of the flagella had basically a purely sinusoidal time course to within +/- 50 cm-1, but a varying amount of asymmetry was present in the distal and the proximal ends of the flagella. This asymmetry in the curvature was related to the radius of the circular path of the sperm. The flagellar waveforms can probably be summarized in simple algebraic functions.
在22摄氏度至6摄氏度的不同温度下，对海胆精子进行了高速电影显微摄影。构建了一套将电视摄像机和视频数字化仪相结合的设备，用于扫描单个鞭毛图像并将鞭毛波形数字化。通过适当的平滑和平均程序，粗糙的数据被微型计算机浓缩为沿着鞭毛的20个点的坐标，这些点相隔2微米。还计算了这些点处鞭毛的曲率。鞭毛位置的坐标精确到约±0.1微米，鞭毛曲率精确到约±50厘米-1。在所有温度下，发现鞭毛的振幅随时间呈纯正弦方式变化，变化幅度在±2%以内。鞭毛的局部曲率在±50厘米-1以内基本呈纯正弦时间进程，但在鞭毛的远端和近端存在不同程度的不对称性。这种曲率的不对称性与精子圆周路径的半径有关。鞭毛波形可能可以用简单的代数函数来概括。
