Brokaw C J
Cell Motil. 1984;4(6):417-30. doi: 10.1002/cm.970040603.
Parameters to describe flagellar bending patterns can be obtained by a microcomputer procedure that uses a set of parameters to synthesize model bending patterns, compares the model bending patterns with digitized and filtered data from flagellar photographs, and uses the Simplex method to vary the parameters until a solution with minimum root mean square differences between the model and the data is found. Parameters for Chlamydomonas bending patterns have been obtained from comparison of shear angle curves for the model and the data. To avoid the determination of the orientation of the basal end of the flagellum, which is required for calculation of shear angles, parameters for sperm flagella have been obtained by comparison of curves of curvature as a function of length for the model and for the data. A constant curvature model, modified from that originally used for Chlamydomonas flagella, has been used for obtaining parameters from sperm flagella, but the methods can be applied using other models for synthesizing the model bending patterns.
描述鞭毛弯曲模式的参数可通过一种微机程序获得，该程序使用一组参数来合成模型弯曲模式，将模型弯曲模式与来自鞭毛照片的数字化和滤波后的数据进行比较，并使用单纯形法来改变参数，直到找到模型与数据之间均方根差最小的解。衣藻弯曲模式的参数是通过比较模型和数据的剪切角曲线获得的。为避免确定鞭毛基部末端的方向（计算剪切角需要此方向），精子鞭毛的参数是通过比较模型和数据的曲率随长度变化的曲线获得的。一种从最初用于衣藻鞭毛的模型修改而来的恒定曲率模型已被用于从精子鞭毛中获取参数，但这些方法也可使用其他用于合成模型弯曲模式的模型来应用。
