Liu Zhibo, Huang Xinshuo, Liu Zhengjie, Zheng Shantao, Yao Chuanjie, Zhang Tao, Huang Shuang, Zhang Junrui, Wang Jizhuang, Farah Shady, Xie Xi, Chen Hui-Jiuan
State Key Laboratory of Optoelectronic Materials and Technologies, School of Electronics and Information Technology; Guangdong Province Key Laboratory of Display Material and Technology, Sun Yat-Sen University, Guangzhou 510006, China.
Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument, School of Biomedical Engineering, Sun Yat-Sen University, Shenzhen 518107, China.
ACS Sens. 2025 Jan 24;10(1):159-174. doi: 10.1021/acssensors.4c01595. Epub 2025 Jan 9.
Gouty arthritis is one of the most common forms of inflammatory arthritis and has brought a significant burden on patients and society. Current strategies for managing gout primarily focus on long-term urate-lowering therapy. With the rapid advancement of point-of-care testing (POCT) technology, continuous monitoring of gout-related biomarkers like uric acid (UA) or inflammatory cytokines can provide rapid and personalized diagnosis for gout management. In this study, a plug-in design of a microneedle electrode array (PIMNA) was developed and integrated into a multi-parameter sensing portable system in combination with embedded circuits and a mobile application. The system enabled real-time, in situ, and dynamic monitoring of biomarkers, including UA, reactive oxygen species (ROS), and pH at gouty joints. The multi-parameter monitoring system demonstrated a wide linear response range, excellent selectivity, stability, reproducibility, and reliable signal transmission performance. In vivo experiments demonstrated the real-time monitoring capability of PIMNA for UA, ROS, and pH, showing the potential to facilitate urate-lowering management and inflammation assessment. Prospectively, the system enables quantitative analysis of the complexity and diversity of gout, presenting promising applications in clinical practice. This work provides a unique strategy with potential for broader applications in gout management and arthritic disease treatment.
痛风性关节炎是最常见的炎症性关节炎形式之一，给患者和社会带来了沉重负担。目前痛风管理策略主要集中在长期降尿酸治疗。随着即时检测（POCT）技术的快速发展，对尿酸（UA）或炎性细胞因子等痛风相关生物标志物的持续监测可为痛风管理提供快速且个性化的诊断。在本研究中，开发了一种微针电极阵列的插件设计（PIMNA），并将其与嵌入式电路和移动应用相结合，集成到一个多参数传感便携式系统中。该系统能够对痛风关节处的生物标志物进行实时、原位和动态监测，包括UA、活性氧（ROS）和pH值。该多参数监测系统显示出宽线性响应范围、优异的选择性、稳定性、重现性以及可靠的信号传输性能。体内实验证明了PIMNA对UA、ROS和pH值的实时监测能力，显示出其在促进降尿酸管理和炎症评估方面的潜力。前瞻性地看，该系统能够对痛风的复杂性和多样性进行定量分析，在临床实践中具有广阔的应用前景。这项工作提供了一种独特的策略，在痛风管理和关节炎疾病治疗中具有更广泛应用的潜力。
