Lin Yun, Su Rui, Huang Yun
Department of Hematology, The First Affiliated Hospital of Xiamen University and Institute of Hematology, School of Medicine, Xiamen University, Xiamen, 361003, China.
J Fluoresc. 2025 Jan 9. doi: 10.1007/s10895-024-04092-y.
Diffuse large B-cell lymphoma (DLBCL) is a highly aggressive diffuse malignant proliferative disease of the lymphatic system. Patients usually present with progressive lymph node enlargement and/or extra-lymph node lesions and require early treatment upon diagnosis. Most of the patients are in stage III or IV at the time of diagnosis and about 40% of the patients are difficult to cure. In this study, we developed a novel drug delivery platform using poly(ε-caprolactone)-tetraphenylethylene (PCL-TPE). This fluorescent polymer, combined with a solid-state dye, exhibits temperature-dependent fluorescence. We loaded the synthesized ethyl 2-amino-4-(4-methylthiophen-2-yl)-5,6,7,8-tetrahydro-2 H-chromene-3-carboxylate (compound 1) onto PCL-TPE, creating PCL-TPE@1. By treating human lymphoma cells, we found that PCL-TPE@1 was able to effectively reduce the migratory ability of lymphoma cells by inhibiting the expression of PRMT6. This platform is easy to synthesize, offers thermo-responsive fluorescence, flexibility, and biocompatibility, making it suitable for biomedical drug delivery and smart devices.
弥漫性大B细胞淋巴瘤（DLBCL）是一种高度侵袭性的淋巴系统弥漫性恶性增殖性疾病。患者通常表现为进行性淋巴结肿大和/或淋巴结外病变，诊断后需要尽早治疗。大多数患者在诊断时处于III期或IV期，约40%的患者难以治愈。在本研究中，我们开发了一种使用聚（ε-己内酯）-四苯乙烯（PCL-TPE）的新型药物递送平台。这种荧光聚合物与固态染料结合，表现出温度依赖性荧光。我们将合成的2-氨基-4-（4-甲基噻吩-2-基）-5,6,7,8-四氢-2H-色烯-3-羧酸乙酯（化合物1）负载到PCL-TPE上，制成PCL-TPE@1。通过处理人淋巴瘤细胞，我们发现PCL-TPE@1能够通过抑制PRMT6的表达有效降低淋巴瘤细胞的迁移能力。该平台易于合成，具有热响应荧光、灵活性和生物相容性，适用于生物医学药物递送和智能设备。
