Doan Vu Hoang Minh, Mondal Sudip, Vo Thi Mai Thien, Ly Cao Duong, Vu Dinh Dat, Nguyen Van Tu, Park Sumin, Choi Jaeyeop, Oh Junghwan
Industry 4.0 Convergence Bionics Engineering, Department of Biomedical Engineering, Pukyong National University, Busan 48513, Republic of Korea; New-senior Healthcare Innovation Center (BK21 Plus), Pukyong National University, Busan 48513, Republic of Korea.
New-senior Healthcare Innovation Center (BK21 Plus), Pukyong National University, Busan 48513, Republic of Korea.
Colloids Surf B Biointerfaces. 2022 Jun;214:112458. doi: 10.1016/j.colsurfb.2022.112458. Epub 2022 Mar 11.
Multifunctional nanomaterials developed from hydroxyapatite (HAp) with enhanced biological characteristics have recently attracted attention in the biomedical field. The goal of this study is to investigate the potential applications of cobalt-doped HAp (Co-HAp) in the biomedical imaging and therapeutic applications. The co-precipitation approach was used to substitute different molar concentrations of Ca ions with cobalt (Co) in HAp structure. The synthesized Co-HAp nanoparticles were studied using various sophisticated techniques to verify the success rate of the doping method. The specific crystal structure, functional groups, size, morphology, photoluminescence property, and thermal stability of the Co-HAp nanoparticles were analyzed based on the characterization results. The computational modelling of doped and undoped HAp reveals the difference in crystal structure parameters. The cytotoxicity study (MTT assay and AO/PI/Hoechst fluorescence staining) reveals the non-toxic characteristics of Co-HAp nanoparticles on MDA-MB-231 breast cancer cell lines. The DOX was loaded onto Co-HAp, showing the maximum drug loading capacity for 2.0 mol% Co-HAp. Drug release was estimated in five different pH environments with various time intervals over 72 h. Furthermore, 2.0 mol% Co-HAp shows excellent fluorescence sensitivity with FITC-conjugated MDA-MB-231 cell lines. These results suggest that cobalt improved the fluorescence intensity of FITC-labeled HAp nanoparticles. This work highlights the promising application of Co-HAp nanoparticles with significant enhanced fluorescence activity for imaging-guided drug delivery system.
由具有增强生物特性的羟基磷灰石（HAp）开发的多功能纳米材料最近在生物医学领域引起了关注。本研究的目的是研究钴掺杂羟基磷灰石（Co-HAp）在生物医学成像和治疗应用中的潜在应用。采用共沉淀法在HAp结构中用钴（Co）替代不同摩尔浓度的钙离子。使用各种精密技术对合成的Co-HAp纳米颗粒进行研究，以验证掺杂方法的成功率。根据表征结果分析了Co-HAp纳米颗粒的具体晶体结构、官能团、尺寸、形态、光致发光性能和热稳定性。掺杂和未掺杂HAp的计算模型揭示了晶体结构参数的差异。细胞毒性研究（MTT法和AO/PI/Hoechst荧光染色）揭示了Co-HAp纳米颗粒对MDA-MB-231乳腺癌细胞系的无毒特性。将阿霉素负载到Co-HAp上，2.0 mol%的Co-HAp显示出最大载药量。在72小时内，在五个不同pH环境下，以不同时间间隔估计药物释放。此外，2.0 mol%的Co-HAp对FITC偶联的MDA-MB-231细胞系表现出优异的荧光敏感性。这些结果表明钴提高了FITC标记的HAp纳米颗粒的荧光强度。这项工作突出了具有显著增强荧光活性的Co-HAp纳米颗粒在成像引导药物递送系统中的应用前景。
