Sugawara Ichiro, Kawahara Yosuke, Takayasu Lena, Isshi Kimio, Kato Masayuki, Ono Shingo, Hara Yuko, Futakuchi Toshiki, Furuhashi Hiroto, Kurokawa Rina, Sumiyama Kazuki, Suda Wataru
Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan.
Department of Endoscopy, The Jikei University School of Medicine, Tokyo, Japan.
PLoS One. 2025 Jan 9;20(1):e0317230. doi: 10.1371/journal.pone.0317230. eCollection 2025.
Biliary stent occlusion is due, in part, to biofilm formation by bacteria. However, previous culture-based approaches may not have revealed all microorganisms on the surface. Twenty-seven patients underwent endoscopic retrograde biliary drainage for the removal or replacement of plastic biliary stents. We analyzed occlusion severity using image-analyses of a longitudinal section of the biliary stent and evaluated the microbial profile of sludge deposition inside the stents using 16S rRNA sequencing with a MiSeq Illumina platform. We then evaluated the association of microbial profiles with the duration of stent placement and stent occlusion severity. Actinobacteria and Synergistetes were much more abundant in occluded stents compared with non-occluded stents. An abundance of Bifidobacterium spp. and OTU00006 Bifidobacterium animalis (100%) correlated with stent occlusion severity (rho, 0.62; p<0.001; and 0.42; p = 0.03, respectively), and this relationship remained after adjusting for the duration of stent placement (p = 0.03 and 0.05, respectively). The genus Bifidobacterium and Bifidobacterium animalis were associated with the degree of occlusion in plastic biliary stents.
胆管支架闭塞部分归因于细菌形成生物膜。然而，以往基于培养的方法可能并未揭示表面所有的微生物。27例患者接受了内镜逆行胆管引流术以取出或更换塑料胆管支架。我们通过对胆管支架纵切面的图像分析来分析闭塞严重程度，并使用Illumina MiSeq平台的16S rRNA测序评估支架内污泥沉积物的微生物谱。然后我们评估了微生物谱与支架放置时间及支架闭塞严重程度之间的关联。与未闭塞的支架相比，放线菌门和互养菌门在闭塞支架中更为丰富。大量的双歧杆菌属和OTU00006动物双歧杆菌（100%）与支架闭塞严重程度相关（斯皮尔曼相关系数分别为0.62；p<0.001；以及0.42；p = 0.03），并且在调整支架放置时间后这种关系依然存在（分别为p = 0.03和0.05）。双歧杆菌属和动物双歧杆菌与塑料胆管支架的闭塞程度相关。
