Osman Henigul, Wang Fan, Zou Guoming, Zhang Dong, Bai Xi, Jiang Tao, Wang Yingbo
College of Chemical Engineering, Xinjiang Normal University, 102 Xinyi Road, Urumqi 830054, P.R. China.
Department of Orthopaedics, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, Shanghai 200025, P.R. China.
Colloids Surf B Biointerfaces. 2025 May;249:114489. doi: 10.1016/j.colsurfb.2024.114489. Epub 2024 Dec 30.
Infection and insufficient osseointegration are the primary factors leading to the failure of titanium-based implants. Surface coating modifications that combine both antibacterial and osteogenic properties are commonly employed strategies. However, the challenge of achieving rapid antibacterial action and consistent osteogenesis with these coatings remains unresolved. In this study, a functional composite coating (PDA/PPy@Cu/Dex) was prepared on titanium surfaces using layer-by-layer self-assembly and electrochemical deposition techniques. The hydroxyl groups grafted by polydopamine's (PDA) self-polymerization and the enhanced conductivity and uniform electric field distribution provided by polypyrrole (PPy) allowed for the even dispersion of copper nanoparticles and dexamethasone (Dex) on the titanium surface. This synergistically coupled the photothermal ion antibacterial properties of copper nanoparticles with the osteogenic promotion of dexamethasone. In vitro antibacterial experiments revealed that the heat generated by photothermal effects and reactive oxygen species enhanced the antibacterial activity of copper ions, reducing the antibacterial time to six h and achieving antibacterial enhancement. In vitro cell experiments showed that the long-term slow release of copper ions and dexamethasone enhanced the osteogenic differentiation of stem cells, thereby achieving osteogenic benefits. Moreover, in vivo toxicity experiments demonstrated that the composite coating had no adverse effects on normal tissues. Therefore, the antibacterial and osteogenic enhancement strategy for titanium surfaces presented in this study offers a new potential approach for preventing implant-associated infections.
感染和骨整合不足是导致钛基植入物失败的主要因素。结合抗菌和成骨特性的表面涂层改性是常用策略。然而，通过这些涂层实现快速抗菌作用和持续成骨的挑战仍未解决。在本研究中，采用层层自组装和电化学沉积技术在钛表面制备了一种功能性复合涂层（PDA/PPy@Cu/Dex）。聚多巴胺（PDA）自聚合接枝的羟基以及聚吡咯（PPy）提供的增强导电性和均匀电场分布，使得铜纳米颗粒和地塞米松（Dex）能够在钛表面均匀分散。这协同耦合了铜纳米颗粒的光热离子抗菌性能与地塞米松的成骨促进作用。体外抗菌实验表明，光热效应产生的热量和活性氧增强了铜离子的抗菌活性，将抗菌时间缩短至6小时并实现了抗菌增强。体外细胞实验表明，铜离子和地塞米松的长期缓慢释放增强了干细胞的成骨分化，从而实现了成骨益处。此外，体内毒性实验表明复合涂层对正常组织无不良影响。因此，本研究中提出的钛表面抗菌和成骨增强策略为预防植入物相关感染提供了一种新的潜在方法。
