Jiang Xiaodan, Guo Jiabao, Zhang Yuhan, Zuo Hubao, Bao Yuanqi, Liu Nian, Guo Mingna, Wu Ronghai, Chen Qiang
State Key Laboratory of Solidification Processing, School of Materials Science and Engineering, Northwestern Polytechnical University, Xi'an 710072, China.
School of Mechanics, Civil Engineering and Architecture, Northwestern Polytechnical University, Xi'an 710072, China.
J Colloid Interface Sci. 2025 Mar 15;682:1116-1126. doi: 10.1016/j.jcis.2024.11.244. Epub 2024 Dec 6.
Porous architecture of titanium implants offers significant advantages in promoting osseointegration and mitigating the "stress shielding" effect. However, challenges remain in enhancing vascularization and preventing infection, especially given the complexities of modifying the intricate surface structure of porous titanium (PT). This study introduces a novel surface modification technique of PT using anti-gravity perfusion electrophoretic deposition (EPD) technique to fabricate antibacterial coatings containing silver (Ag) and copper (Cu) co-doped mesoporous silica nanoparticles (Ag-Cu@MSN) and chitosan binder on the surface of PT. The developed coating exhibits rapid and stable photothermal response to 808 nm near-infrared (NIR) light, leading to a localized temperature rise and triggered release of Ag and Cu ions for synergistic bactericidal efficacy against both E. coli and S. aureus. In vitro studies further demonstrated that the coated PT significantly promoted the proliferation of mouse calvaria pre-osteoblast (MC3T3-E1) and human umbilical vein endothelial cells (HUVECs), with a notable increase in HUVECs migration and angiogenesis, attributable to the presence of Cu ions. This innovative EPD-based coating strategy offers a promising avenue for developing uniform, photothermally-activated antibacterial surfaces on PT implants, with substantial implications for advancing clinical bone repair applications.
钛植入物的多孔结构在促进骨整合和减轻“应力屏蔽”效应方面具有显著优势。然而，在增强血管化和预防感染方面仍存在挑战，特别是考虑到修饰多孔钛（PT）复杂表面结构的复杂性。本研究引入了一种新颖的PT表面改性技术，即利用反重力灌注电泳沉积（EPD）技术在PT表面制备含银（Ag）和铜（Cu）共掺杂介孔二氧化硅纳米颗粒（Ag-Cu@MSN）和壳聚糖粘合剂的抗菌涂层。所制备的涂层对808nm近红外（NIR）光表现出快速且稳定的光热响应，导致局部温度升高并触发Ag和Cu离子的释放，从而对大肠杆菌和金黄色葡萄球菌产生协同杀菌效果。体外研究进一步表明，涂覆的PT显著促进了小鼠颅骨前成骨细胞（MC3T3-E1）和人脐静脉内皮细胞（HUVECs）的增殖，HUVECs的迁移和血管生成显著增加，这归因于Cu离子的存在。这种基于EPD的创新涂层策略为在PT植入物上开发均匀的、光热激活的抗菌表面提供了一条有前景的途径，对推进临床骨修复应用具有重要意义。
