Abdel-Rahim M, Ezra D, Peck C, Lazar J
Clin Chem. 1985 Apr;31(4):621-3.
In this micro-scale method for liquid-chromatographic measurement of cimetidine in 250-microL volumes of plasma or gastric fluid, a combination of organic- and aqueous-phase extractions, and protonation of the internal standard and cimetidine, enabled us to detect 2.0 ng of cimetidine on the column. We also used a radial compression module, which reduced the retention times for cimetidine and the internal standard to only 2.78 and 2.00 min. The speed and sensitivity of this method facilitates analysis of large numbers of samples.
在这种用于在250微升血浆或胃液中进行西咪替丁液相色谱测量的微量方法中，有机相和水相萃取相结合，以及内标和西咪替丁的质子化，使我们能够在柱上检测到2.0纳克西咪替丁。我们还使用了径向压缩模块，这将西咪替丁和内标的保留时间分别缩短至仅2.78分钟和2.00分钟。该方法的速度和灵敏度有助于大量样品的分析。
