Lin Zengxiang, Wei Wei, Cai Danling, Shan Wei, Chen Jianye, Yang Yingying, Si Jia, Lu Wangjin, Zhu Guohui, Wu Chaojie, Kuang Jianfei
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Engineering Research Center of Southern Horticultural Products Preservation, Ministry of Education, College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
Food Chem. 2025 Apr 15;471:142770. doi: 10.1016/j.foodchem.2025.142770. Epub 2025 Jan 5.
Sodium hydrosulfide (NaHS), a hydrogen sulfide (H₂S) donor, effectively mitigates chilling injury (CI) in bananas; however, the underlying molecular mechanisms remain unclear. This study demonstrated that NaHS alleviates CI symptoms by activating antioxidant defense systems that reduce oxidative stress induced by CI. Transcriptomic analysis revealed 1003 differentially expressed genes in three sample groups, with enrichment in pathways related to cellular processes, metabolic activity, and secondary metabolite biosynthesis. NaHS treatment significantly upregulated seven ethylene response factors, among which MaERF53L and MaERF121L functioned as transcriptional activators. Further investigation revealed that MaERF53L and MaERF121L directly bind to the promoters of MaPOD3 and MaGSTU18, enhancing their expression as well as the antioxidant enzyme activity. These findings indicate that the activation of antioxidant genes by MaERF53L and MaERF121L is central to NaHS-induced CI mitigation in bananas, providing novel insights into the role of H₂S in reducing CI across horticultural crops.
硫化氢供体硫氢化钠（NaHS）可有效减轻香蕉的冷害（CI）；然而，其潜在的分子机制仍不清楚。本研究表明，NaHS通过激活抗氧化防御系统来减轻冷害症状，该系统可降低冷害诱导的氧化应激。转录组分析揭示了三个样本组中有1003个差异表达基因，这些基因富集于与细胞过程、代谢活性和次生代谢物生物合成相关的途径中。NaHS处理显著上调了七个乙烯反应因子，其中MaERF53L和MaERF121L作为转录激活因子发挥作用。进一步研究表明，MaERF53L和MaERF121L直接结合到MaPOD3和MaGSTU18的启动子上，增强它们的表达以及抗氧化酶活性。这些发现表明，MaERF53L和MaERF121L对抗氧化基因的激活是NaHS减轻香蕉冷害的核心，为硫化氢在减轻园艺作物冷害中的作用提供了新的见解。
