Willemse A, Noordzij A, Rutten V P, Bernadina W E
Clin Exp Immunol. 1985 Feb;59(2):351-8.
Dogs immunized with o-DNCP-BSA or eggs of Toxocara canis developed anaphylactic antibodies other than IgE. The antibodies were heat-resistant at 56 degrees C for 2 hours, not reduced by 2-mercaptoethanol and PCA and P-K reactivity was only observed at 3-4 h sensitized sites of recipient skin. High titres of antigen-specific IgGd were determined by means of an ELISA technique.
用邻二硝基氯苯 - 牛血清白蛋白(o - DNCP - BSA)或犬弓首蛔虫卵免疫的狗产生了除IgE之外的过敏抗体。这些抗体在56℃下2小时耐热,不被2 - 巯基乙醇、PCA还原,并且仅在受体皮肤致敏部位3 - 4小时观察到被动皮肤过敏反应(PCA)和被动 - 即刻反应(P - K)反应。通过酶联免疫吸附测定(ELISA)技术测定了高滴度的抗原特异性IgGd。