Almási Éva D H, Eisenhard Lea, Osbelt Lisa, Lesker Till Robin, Vetter Anna C, Knischewski Nele, Bielecka Agata Anna, Gronow Achim, Muthukumarasamy Uthayakumar, Wende Marie, Tawk Caroline, Neumann-Schaal Meina, Brönstrup Mark, Strowig Till
Department of Microbial Immune Regulation, Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany.
Department of Chemical Biology, Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany.
Nat Commun. 2025 Jan 9;16(1):551. doi: 10.1038/s41467-024-55800-y.
Competition among bacteria for carbohydrates is pivotal for colonization resistance (CR). However, the impact of Western-style diets on CR remains unclear. Here we show how the competition between Klebsiella oxytoca and Klebsiella pneumoniae is modulated by consuming one of three Western-style diets characterized by high-starch, high-sucrose, or high-fat/high-sucrose content. In vivo competition experiments in ampicillin-treated mice reveal that K. oxytoca promotes K. pneumoniae decolonization on all dietary backgrounds. However, mice on the high-fat/high-sucrose diet show reduced pathogen clearance. Microbiome analysis reveals that the combination of Western-style diets and ampicillin treatment synergize in microbiome impairment, particularly noticeable in the presence of high dietary fat content. The diet-independent degradation of ampicillin in the gut lumen by K. oxytoca beta-lactamases facilitates rapid commensal outgrowth, which is required for subsequent pathogen clearance. Our findings provide insights into how diet modulates functional microbiome recovery and K. oxytoca-mediated pathogen elimination from the gut.
细菌之间对碳水化合物的竞争对于定植抗性（CR）至关重要。然而，西式饮食对CR的影响仍不清楚。在这里，我们展示了产酸克雷伯菌和肺炎克雷伯菌之间的竞争是如何通过食用三种西式饮食之一来调节的，这三种西式饮食的特点分别是高淀粉、高蔗糖或高脂肪/高蔗糖含量。在氨苄青霉素处理的小鼠中进行的体内竞争实验表明，在所有饮食背景下，产酸克雷伯菌都能促进肺炎克雷伯菌的去定植。然而，食用高脂肪/高蔗糖饮食的小鼠病原体清除能力降低。微生物组分析表明，西式饮食和氨苄青霉素治疗的组合在损害微生物组方面具有协同作用，在饮食中脂肪含量高的情况下尤为明显。产酸克雷伯菌β-内酰胺酶在肠腔中对氨苄青霉素的非饮食依赖性降解促进了共生菌的快速生长，这是随后清除病原体所必需的。我们的研究结果为饮食如何调节功能性微生物组恢复以及产酸克雷伯菌介导的肠道病原体清除提供了见解。
