Jiang Yingxin Celia, Che Qi, Lu Xinmei, Liu Miao, Ye Yao, Cao Xiang, Li Xushuo, Zhan Yanxia, Dong Xi, Cheng Yunfeng, O'Neill Christopher
Charles Perkins Centre, The University of Sydney, Sydney, NSW, Australia.
Centenary Institute, The University of Sydney, Sydney, NSW, Australia.
Front Endocrinol (Lausanne). 2024 Dec 26;15:1464171. doi: 10.3389/fendo.2024.1464171. eCollection 2024.
It is well acknowledged that lipids assume a critical role in oocyte maturation and early embryonic metabolism, this study aimed to evaluate the relationship between the lipid composition of plasma and follicular fluid (FF), and the consequences of embryonic development. This study compared the lipidomic profiles of paired plasma and FF samples obtained from sixty-five Chinese women who underwent assisted reproductive technology (ART) treatments.
Non-targeted lipidomics analysis.
Results not only indicated similarities in lipid composition between these biofluids, but also revealed a number of unique differences. The biomatrix distinction was found to be primarily driven by lipids belonging to the lysophosphatidylcholines (LPC), phosphatidylethanolamines (PE), ether PE, and triglyceride (TG) classes. In addition, specific species from these subclasses were discovered to be correlated with embryo development outcomes during ART. Notably, the composition of the fatty acyl chains appeared to play a crucial role in these associations. Furthermore, thirteen plasma lipid variables were identified, represented by Phosphatidylcholine 18:014:0 and PE P-18:020:1, which correlated with successful blastocyst formation (BF).
The present study demonstrated that FF has a distinctive lipid composition, setting it apart from plasma; and the association observed with embryonic development underscored an important role of lipid composition in the healthy development of oocytes.
众所周知，脂质在卵母细胞成熟和早期胚胎代谢中起着关键作用，本研究旨在评估血浆和卵泡液（FF）的脂质组成之间的关系，以及胚胎发育的结果。本研究比较了从65名接受辅助生殖技术（ART）治疗的中国女性中获得的配对血浆和FF样本的脂质组学图谱。
非靶向脂质组学分析。
结果不仅表明这些生物流体在脂质组成上具有相似性，还揭示了许多独特的差异。发现生物基质差异主要由属于溶血磷脂酰胆碱（LPC）、磷脂酰乙醇胺（PE）、醚PE和甘油三酯（TG）类别的脂质驱动。此外，发现这些亚类中的特定种类与ART期间的胚胎发育结果相关。值得注意的是，脂肪酰链的组成似乎在这些关联中起着关键作用。此外，确定了13个血浆脂质变量，以磷脂酰胆碱18:014:0和PE P-18:020:1为代表，它们与成功的囊胚形成（BF）相关。
本研究表明，FF具有独特的脂质组成，与血浆不同；并且观察到的与胚胎发育的关联强调了脂质组成在卵母细胞健康发育中的重要作用。
