用于溶解眼晶状体核心（核）蛋白质的新介质。

New medium for solubilizing proteins of the eye lens core (nucleus).

作者信息

Smith A C

出版信息

Comp Biochem Physiol B. 1985;80(2):377-80. doi: 10.1016/0305-0491(85)90222-6.

Abstract

The eye lens core (nucleus) is a practical source of proteins for use as genetic markers. A new, simple extraction solution for these proteins is described. The solution was tested on fish (mullet, rockfish, and barracuda) lens nuclei, which produced weak extracts with other extraction media. A new solution, in addition to producing the strongest extracts, demonstrated desirable qualities relating to electrophoresis and storage. This solution may serve as a general extraction solution for nuclear lens proteins.

摘要

眼晶状体核心（核）是用作遗传标记的蛋白质的一个实用来源。本文描述了一种用于这些蛋白质的新型简单提取溶液。该溶液在鱼类（鲻鱼、岩鱼和梭鱼）晶状体核上进行了测试，这些晶状体核用其他提取介质提取效果不佳。一种新溶液除了能产生最强的提取物外，还在电泳和储存方面表现出理想的特性。这种溶液可作为晶状体核蛋白的通用提取溶液。