线粒体膜冷冻破坏导致的线粒体特性功能变化。I. 冻融对完整肝线粒体琥珀酸 - 铁氰化物还原酶的影响

Functional changes in mitochondrial properties as a result of their membrane cryodestruction. I. Influence of freezing and thawing on succinate-ferricyanide reductase of intact liver mitochondria.

作者信息

Tsvetkov T, Tsonev L, Meranzov N, Minkov I

出版信息

Cryobiology. 1985 Feb;22(1):47-54. doi: 10.1016/0011-2240(85)90007-0.

Abstract

The interaction of the succinate dehydrogenase complex of rat liver mitochondria with an artificial electron acceptor (K3Fe(CN)6), impermeable to the mitochondrial membrane as an index of a cryoinjury is investigated. It is shown that the freeze-thawing stimulates succinate-ferricyanide reductase (SFCR) activity of intact mitochondria. The increase of the freezing and thawing rates leads to a decrease in the released SFCR activity. The released SFCR activity after low-temperature treatment is a consequence of a nonspecific change in membrane ferricyanide permeability. The released SFCR activity decreases as the freezing and thawing rates increase.

摘要

研究了大鼠肝线粒体琥珀酸脱氢酶复合物与一种线粒体内膜不可渗透的人工电子受体（铁氰化钾，K3Fe(CN)6）的相互作用，以此作为冷冻损伤的指标。结果表明，冻融处理可刺激完整线粒体的琥珀酸 - 铁氰化物还原酶（SFCR）活性。冷冻和解冻速率的增加会导致释放的SFCR活性降低。低温处理后释放的SFCR活性是膜铁氰化物通透性非特异性变化的结果。随着冷冻和解冻速率的增加，释放的SFCR活性降低。